利用基因枪法将Tagsk1基因导入敏盐小麦成熟胚愈伤组织提高其耐盐性的研究  

Introduce Tagsk1 into Salt-sensitive Callus to Improve the Capacity of Salt-tolerance by Micropartical Bombardment

作　　者：徐涛[1] 赵宝存[1] 葛荣朝[1] 沈银柱[1] 黄占景[1] 

机构地区：[1]河北师范大学生命科学学院,石家庄050016

出　　处：《生物工程学报》2006年第2期211-214,共4页Chinese Journal of Biotechnology

基　　金：国家转基因专项(No.2004JYA01);国家自然科学基金(No.30070471);河北省自然科学基金(No.301103;No.2005000164)。~~

摘　　要：以Tagsk1(TriticumasetiumL.glycogen synthase kinase1)基因的cDNA的碱基序列为基础,设计特异引物由小麦耐盐突变体RH8706-49基因组DNA进行扩增后,得到来自于基因组的Tagsk1基因。采用基因枪法,利用携带该基因的双元表达载体pBI121-gsk1转化敏盐小麦H8706-34和中国春的成熟胚愈伤组织,经Kanamycin和0.5%NaCl筛选获得耐盐愈伤组织。这些被转化的愈伤组织表现出较高的耐盐性,并且能够在含盐培养基上进一步分化出根和芽。The Tagsk1（ Triticum asetium L. glycogen synthase kinase 1）gene derived from the genome of wheat salt-tolerance mutant RH8706-49 was cloned by PCR. The special primers designed according to full length cDNA sequence of Tagsk1 （AF525086）. A binary expression vector pBI121-gsk1 containing Gus and Tagsk1 was constructed, And pBI121-gsk1 was introduced into the callus induced from mature embryos of salt-sensitive wheat H8706-34 and cv. China Spring by particle bombardment. The transformed callus were screened by Kanamycin and 0.5 % NaCl, The salt-tolerance callus were obtained, which showed higher ability of salt-tolerance and could diffirentiate roots and buds on the medium containing 0.5 % NaCl.

关 键 词：基因枪 转化 小麦 成熟胚愈伤组织 Tagsk1 

分 类 号：S512.1[农业科学—作物学]