拟南芥VHA-c3基因的特异性表达和调节  

Regulation and Tissue Specific Expression of VHA-c3 of Arabidopsis Thaliana

作　　者：徐萍[1] 李小方[1] 曾卫军[1] 王水平[1] 

机构地区：[1]华东师范大学生命科学学院,上海200062

出　　处：《华东师范大学学报（自然科学版）》2006年第2期98-104,111,共8页Journal of East China Normal University(Natural Science)

基　　金：国家自然科学基金资助项目(30460018)

摘　　要：利用RT-PCR技术检测VHA-c基因在拟南芥中的表达，结果表明VHA-c3基因在拟南芥的果荚、花、叶、茎和根中都有表达，但是，在叶中的表达量远远高于其它的组织．以GUS基因作为报告基因构建了不同长度的VHA-c3基因启动子缺失突变体，利用农杆菌介导的瞬时表达系统检测GUS基因的表达，研究发现在VHA-c3基因起始密码子上游2812～2234bp之间的区域内存在着控制VHA-c3基因高表达的转录调控元件．Gene expression of VHA c was detected in Arabidopsis silique, flower, leaf, bolt, root. However, the expression level of VHA-c3 was much higher in leaves than in the other tissues. Analysis of the putative promoter of VHA-c3 was carried out in tobacoo leave system. A series of the promoter mutants were constructed in which various extents of VHA-c3 promoter were placed upstream of the GUS gene in vector pC1305.1. The expression of GUS was analyzed by Agrobacterium mediated transient expression system. It was found that a 2 812 bp promoter region has high gene expression capacity. And there is a high potential key cis element for leafspecific expression between 2 812-2 234 bp region.

关 键 词：V-ATPASE C亚基 VHA-c3基因 启动子 

分 类 号：Q945[生物学—植物学]