川芎RP-HPLC指纹图谱的建立及其质量研究  

Establishment of Chromatographic Fingerprint and Quality Assessment of Rhizoma Chuanxiong by HPLC

作　　者：宋金春[1] 胡传芹[2] 曾俊芬[2] 李玉琴[2] 

机构地区：[1]武汉大学人民医院药学部,武汉430060 [2]武汉大学药学院

出　　处：《中国药师》2006年第4期297-301,共5页China Pharmacist

基　　金：湖北省科技攻关项目(编号:2003AA302B02 2004AA302B09)

摘　　要：目的:用高效液相色谱法建立川芎的指纹图谱分析方法,控制川芎药材的质量。方法:采用梯度洗脱的方法进行色谱分离,使用“计算机辅助相似度评价软件”进行数据处理,对不同批药材指纹图谱的相似度进行比较分析。结果:共有10个色谱峰(RSD小于3％,n=10),不同批的川芎药材的指纹图谱相似度较好。结论:采用HPLC方法控制药材的指纹图谱,方法重现性好,用于川芎的质量评价切实可行,可靠、简便,为提高川芎质量控制标准提供参考。不同批的川芎药材化学组成相似,其相对比例较稳定。Objective： To establish a chromatographic fingerprint and quality control of Chuanxiong by RP-HPLC. Method： The gradient elution mode was applied in chromatographic separation, and data were analysed by ＂Computer Aided Similarity Evaluation＂ software to compare the quality of Ligusticum chuanxiong Hort samples from different batches. Result： Ten peaks （ RSD is less than 3% ,n=10） were common and samples of different batches were of high similarity in fingerprint graphics. Conclusion： The RP-HPLC fingerprint method is repeatable, feasible in analysis of Ligusticum chuanxiong Hort and provides a reference standard for the quality control of Ligusticum chuanxiong Hort. Chemical components in Ligusticum chuanxiong Hart from different batches are similar, and their relative ratio between each other are stable.

关 键 词：川芎 RP—HPLC 指纹图谱 

分 类 号：TQ460.72[医药卫生—药物分析学]