血浆凝固酶阴性葡萄球菌快速“两步一补”鉴定方法  

"Two steps with one supplement" method for rapid identification of Coagulase-Negative Staphylococci

作　　者：邓光荣[1] 田维珍[2] 蔡忠祥[1] 陈芳[1] 

机构地区：[1]湖北荆州市第二人民医院,湖北荆州434000 [2]湖北中医药高等专科学校,湖北荆州434020

出　　处：《中国现代医学杂志》2006年第6期899-901,共3页China Journal of Modern Medicine

摘　　要：目的研究血浆凝固酶阴性葡萄球菌(CNS)的生化反应特性,建立一种血浆凝固酶阴性葡萄球菌快速“两步一补”鉴定方法。方法对临床分离的CNS菌株,运用“两步一补”法进行鉴定,然后随机抽取一定量的菌株,经双盲法用法国梅里埃APIStaph试条进行复核。结果该方法4h内能鉴定出54.8%的CNS,24h能鉴定出96.3%的CNS,48小时内能鉴定出全部CNS。随机抽取的80株CNS经过APIStaph试条进行复核,符合率100%。结论“两步一补”法鉴定CNS具有快速简单、准确可靠及成本低廉等优点,更适合基层医院推广应用。[Objective] To study biochemical characteristics of Coagulase-Negative Staphylococci （CNS） and develop a ＂two steps with one supplement＂ method for rapid identification of CNS species. [Method] The clinical Coagulase-Negative Staphylococci isolates were identified firstly by the ＂two steps with one supplement＂ method. From them a certain number of identified species were chosen randomly to be checked again using API Staph （bioMerieux, France） method. [Results] Using the ＂two steps with one supplement＂ method 54.8% of the CNS isolates were identified in 4 h, 96.3% were identified in 24 h and 100% in 48 h. The result of the 80 isolates identified again by API Staph method was 100% in agreement with previous. [Conclusion] The ＂two steps with one supplement＂ method for identification of CNS is reliable, simple rapid, and inexpensive. It is therefore advantageous to be used widely in common hospitals.

关 键 词：凝固酶阴性葡萄球菌 快速鉴定 

分 类 号：R447[医药卫生—诊断学]