MyoD基因诱导大鼠心脏成纤维细胞分化为成肌细胞的实验研究  

Experimental studies on the differentiation of rat cardiac fibroblasts into myoblasts induced by MyoD gene in vitro

作　　者：周秀娟[1] 黄峻[1] 姚堃[2] 周锋[2] 马春玲[2] 

机构地区：[1]南京医科大学第一附属医院心脏科,江苏南京210029 [2]南京医科大学微生物学与免疫学系,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2006年第4期238-241,F0002,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金资助项目(30370575)

摘　　要：目的:探讨MyoD基因诱导大鼠心脏成纤维细胞分化为成肌细胞的可能性。方法:将MyoD基因重组腺病毒载体转染大鼠心脏成纤维细胞,观察转染后大鼠心脏成纤维细胞形态的变化;用RT-PCR和Westernblot方法检测MyoD和肌细胞生成素的表达;免疫组化检测骨骼肌肌球蛋白和结蛋白的表达。结果:MyoD基因转染后的大鼠心脏成纤维细胞形态和排列方式发生明显变化;RT-PCR可检测出转染后细胞表达MyoD;Westernblot结果表明转染后细胞表达MyoD和肌细胞生成素;免疫组化检测骨骼肌肌球蛋白和结蛋白表达均为阳性。结论:MyoD基因可诱导体外培养的大鼠心脏成纤维细胞分化为成肌细胞,为进一步研究MyoD对心肌损伤的修复作用奠定了基础。Objective：To explore the possibility of MyoD gene inducing rat cardiac fibroblasts to differentiate into myoblasts in vitro. Methods：The cultured rat cardiac fibroblasts were transfected with the recombinant adenoviral vector carrying MyoD gene and the changes of these cells morphology were observed.The expression of MyoD and myogenin in the transfected cells was detected by RT-PCR and Western blot. Immunohistochemical methods were used to measure the expression of skeletal myosin and desmin in these cells. Results：The morphology and alignment of the transfected cells changed obviously. These cells were detected out the expression of MyoD by RT-PCR,and the expression of MyoD and myogenin by Western blot. Immunohistochemical methods indicated the expression of skeletal myosin and desmin respectively in these cells. Conelusion：Myoblasts （skeletal muscle）could be differentiated from cultured rat cardial fibroblasts under the induction of MyoD gene,thus providing an experimental basis for studying MyoD gene on restoring myocardium injury.

关 键 词：心脏成纤维细胞 成肌细胞 MyoD基因 诱导 

分 类 号：R322.11[医药卫生—人体解剖和组织胚胎学]