不同表型幽门螺杆菌对幽门螺杆菌相关性胃炎和胃癌组织p53表达的影响  

Effect of different Helicobacter pylori phenotype on p53 expression in Helicobacter pylori associated gastritis and gastric cancer

作　　者：朱苏敏[1] 黄光明[1] 张发明[2] 卢瑗瑗[2] 季国忠[1] 祝金泉 范志宁[1] 浦福兴[1] 

机构地区：[1]南京医科大学消化内镜研究所,江苏南京210011 [2]第四军医大学西京医院肿瘤生物学国家重点实验室 [3]南昌大学消化病研究所,江西南昌330006

出　　处：《南京医科大学学报（自然科学版）》2006年第4期253-256,F0003,共5页Journal of Nanjing Medical University(Natural Sciences)

摘　　要：目的:探讨不同表型幽门螺杆菌(Hp)对Hp相关性胃炎和胃癌病变及其p53表达的影响。方法:对137例Hp(+)慢性胃炎(CG)、消化性溃疡及胃癌患者和34例Hp(-)患者用Westernblot检测Hp细胞毒素相关蛋白(CagA)、空泡毒素(VacA)、尿素酶(Urease)及其亚型。评价Hp(+)CG中慢性炎症(CI)、多形核活动性(PA)、Hp定植密度(DH)的组织学等级。用免疫组化检测p53表达,以H-score方法评价。结果:①Hp128kuCagA、116kuCagA、95kuVacA、91kuVacA、30kuUreA表达在轻度CI组的比例低于中、重度CI组(P均<0.05),95kuVacA、91kuVacA和30kuUreA表达在无PA组的比例低于有PA组(P均<0.05)。②Hp(+)组P53Hscore均高于Hp(-)CG组(P=0.002)。在Hp+CG中,中重度CI组P53Hscore均高于轻度组(P=0.025),而在Hp(-)CG组中,差异无显著性。③Hp+胃癌中,未发现任何独立表型与CG和胃癌的53Hscore有关(P>0.05)。结论:Hp多种毒力因子对胃黏膜CI程度加重、PA的发生和发展起促进作用,Hp感染及DH增加均促进p53表达无关;未见Hp任何独立表型与53表达有关,提示Hp可能通过多种毒力因素及此研究未提及的其他因素的综合作用促进p53过度表达。Objective： To expolre the effect of different Helicobacter pylori （Hp） phenotype on p53 expression in Hp associated gastritis and cancer lesions. Methods：One hundred and thirty-seven patients （with Hp infection） with chronic gastritis（CG）,peptic ulcer or gastric cancer and 34 patients without Hp infection were observed.Western blotting was used to detect the phenotype of Hp Cytotoxin-associated protein （CagA）,vacuolating cytotoxin （VacA）,urease and their sub-phenotypes.In Hp-associated gastritis,histological grade of chronic inflammation （CI）,polymorphonuclear neutrophil acativity （PA） and density of Hp （DH） were scored according to the Updated Sydney System.Immunohistochemistry and H-score were used to detect and evaluate p53 expression. Results：① Expression rate of Hp 128 ku CagA,116 ku CagA,95 ku VacA,91 ku VacA and 30 ku UreA in group of mild grade of CI were lower than those of moderate-marked group （P 〈 0.05 ,respectively）;Hp infection rate and expression rate of 95 ku VacA,91 ku VacA and 30 ku UreA in group of mild grade of PA were lower than those in moderate-marked group（P 〈 0.05 ,respectively）.②p53 Hscore in Hp positive CG group was higher than that of Hp negative CG group （P = 0.002）.And p53 Hscore in group of moderate-marked grade of CI of Hp positive CG group was higher than that of mild group （P = 0.025）,while no significance was found in Hp negative CG group （P 〉 0.05）.③ No significant relationship between any single specific phenotype and p53 Hscore in CG or gastric cancer was found （P 〉 0.05）. Conclusion：Hp infection and their density seem to prompt the increase of grade CI and PA development,whereas the density of Hp isn＇t related to p53 overexpression.

关 键 词：幽门螺杆菌 表型 慢性胃炎 胃癌 P53蛋白 

分 类 号：R378.99[医药卫生—病原生物学]