从聚丙烯酰胺凝胶中回收DNA片段直接PCR的简易方法  

A Simple Method for Extracting DNA from Polyacrylamide Gels and Amplifying DNA Directly

作　　者：倪星群[1] 郭景元[1] 

机构地区：[1]中山医科大学法医物证学教研室

出　　处：《法医学杂志》1996年第1期28-29,共2页Journal of Forensic Medicine

摘　　要：将聚丙烯酸胺凝胶中ＤＮＡ片段于ＰＣＲ缓冲液中电洗脱，加入ＰＣＲ反应试剂，直接扩增目的ＤＮＡ作进一步分析，ＤＮＡ片段回收效率为０．８９±０．０５，本方法简便、快速。In PCR buffer, the DNA was recoveted from polyacrylamide gels by electroelution. The recovery efficiency was 0. 89± 0. 05. Added the PCR reagents,the DNA was directly amplified and the future analysis was performed. This method is easy, quick, inexpensive and highly efficient.

关 键 词：法医物证学 DNA 聚丙烯酰胺凝胶 聚合酶链反应 

分 类 号：Q7－3[生物学—分子生物学] R89[医药卫生—法医学]