Promoter trapping in Magnaporthe grisea  

作　　者：刘小红 卢建平 王教瑜 闵航 林福呈 

机构地区：[1]Biotechnology Institute, School of Agriculture and Biotechnology, Zhejiang University,Hangzhou 310029, China School of Life Sciences, Zhejiang University, Hangzhou 310029, China [2]School of Life Sciences, Zhejiang University,Hangzhou 310029, China [3]Biotechnology Institute, School of Agriculture and Biotechnology, Zhejiang University,Hangzhou 310029, China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2006年第1期28-33,共6页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：Project supported by the National Natural Science Foundation of China (Nos. 30270049 and 30470064) and the Hi-Tech Research and Development Program (863) of China (No. 2002AA245041)

摘　　要：Application of promoter trapping based on transformation in Magnaporthe grisea is reported in this paper. Two promoter-trapping vectors, designated as pCBGFP and pEGFPHPH, were constructed and transformed into protoplasts of M. grisea. A library of 1077 transformants resistant to hygromycin B was generated. Of which, 448 transformants were found to express eGFP gene in different structures of M. grisea. Three transformants grew slowly, 5 transformants decreased in conidiation and 7 transformants reduced in pathogenicity greatly among these 448 transformants. Eleven transformants were checked by genomic southern blot randomly, and 9 of which were single-copy insertions. The promoter trapping technique has been applied successfully in M. grisea and can be used as a tool for functional genomic analysis.Application of promoter trapping based on transformation in Magnaporthe grisea is reported in this paper. Two promoter-trapping vectors, designated as pCBGFP and pEGFPHPH, were constructed and transformed into protoplasts of M. grisea. A library of 1077 transformants resistant to hygromycin B was generated. Of which, 448 transformants were found to express eGFP gene in different structures ofM. grisea. Three transformants grew slowly, 5 transformants decreased in conidiation and 7 transformants reduced in pathogenicity greatly among these 448 transformants. Eleven transformants were checked by genomic southern blot randomly, and 9 of which were single-copy insertions. The promoter trapping technique has been applied successfully in M. grisea and can be used as a tool for functional genomic analysis.

关 键 词：Promoter trapping Green fluorescent protein Magnaporthe grisea 

分 类 号：Q949.32[生物学—植物学]