双黄连粉针剂及其药物血清体外抗HIV-1作用  

Effect of Shuanghuanglian Injection and Its Drug Serum Against HIV-1 In Vitro

作　　者：曾祥凤[1] 曾耀英[1] 林长乐[1] 赵令斋[1] 李海仙[1] 李莉平[1] 

机构地区：[1]暨南大学组织移植与免疫学教育部重点实验室,广东广州510632

出　　处：《中山大学学报（医学科学版）》2006年第2期144-147,156,共5页Journal of Sun Yat-Sen University:Medical Sciences

基　　金："十五"国家科技攻关计划中医药治疗艾滋病专项基金(2004BA719A09-03)

摘　　要：【目的】探讨双黄连药物及其药物血清在体外是否有抗HIV-1作用。【方法】采用荧光染料CalcienAM标记HIV感染的慢性H9细胞(H9/HIV-1ⅢB)分别与不同稀释浓度的双黄连药物和药物血清作用后,与靶细胞MT-2细胞混合培养,观察MT-2和H9/HIV-1ⅢB融合细胞的变化;MTT法检测药物对HIV感染细胞的保护作用;HIV-1p24抗原试剂盒检测药物作用后的细胞培养上清p24抗原含量的变化。【结果】双黄连粉针剂及其药物血清能够抑制MT-2和H9/HIV-1ⅢB细胞融合,能够保护HIV感染细胞的死亡,减少HIV-1p24的表达。【结论】双黄连粉针剂及其药物血清在体外具有抗HIV-1的作用。[Objective] To investigate the inhibitory effect of Shuanghuanglian （SHL） injection on human irarannedeficiency virus type 1 （HIV-1） infection in vitro. [Methods] Calcein-AM stained H9/HIV-1Ⅲ B cells were treated with serially diluted SHL injection and its drug serum respectively, then mixed with target cells MT-2. HIV-1 mediated cell fusion was observed under a fluorescent microscope. The protective effect of SHL on HIV-1-infected cells was determined by MTT. Viral replication was evaluated by measuring the level of p24 antigen in culture supematants by ELISA. [Results] SHL injection and its drug serum could inhibit the fusion of MT-2 and H9/HIV-1ⅢB cells, could protect HIV-1 infected cells from death, and decrease p24 antigen release. [Conclusion] SHL injection and its drug serum can reduce HIV-1 infection in vitro.

关 键 词：双黄连 HIV-1 细胞融合 药物血清 

分 类 号：R373.3[医药卫生—病原生物学]