PCOS患者卵泡基底膜蛋白的免疫组化研究  

Immunohistochemistry of Follicular Basic Membrane Components and Contents in Patients with Polycystic Ovary Syndrome

作　　者：冯淑英[1] 邝健全[1] 余妙真[1] 杨冬梓[1] 翁梅英[1] 李琳[1] 

机构地区：[1]中山大学附属第二医院妇产科,广东广州510120

出　　处：《中山大学学报（医学科学版）》2006年第2期161-164,共4页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：卫生部资金资助项目(D000095007)

摘　　要：【目的】探讨多囊卵巢综合征(PCOS)卵巢细胞外基质代谢是否存在异常。【方法】收集PCOS卵巢组织20例作为PCOS组,正常妇女卵巢组织23例作为对照组,用SP免疫组化法比较两组不同期别卵泡基底膜的Ⅳ型胶原、层连接蛋白及串珠样蛋白聚糖3种主要蛋白组分的表达。【结果】两组原始卵泡基底膜3种主要蛋白组分的表达相似;PCOS组初级卵泡基底膜的Ⅳ型胶原、层连接蛋白的表达明显多于对照组(P<0.05);PCOS组早期次级卵泡及囊状卵泡基底膜的Ⅳ型胶原、层连接蛋白及串珠样蛋白聚的表达明显多于对照组(P<0.05)。【结论】PCOS发育中卵泡基底膜的构成成分表达增多,表明PCOS卵巢细胞外基质代谢异常。[Objective] To explore whether the metabolism of ovarian extra cellular matrix is abnormal in the patients with polycystic ovary syndrome （PCOS）. [Methods] Twenty cases of ovarian tissues from the women with PCOS were enrolled as study group and another 23 eases of ovarian tissues in normal women were selected as control group. S-P immunohistochemical method was adopted to detect the contents of collagenⅣ, laminin, and perlecan in follicular basic membrane in different periods. [Result] The contents of collagenⅣ and laminin in basic membrane of primordial follicles were similar in both groups （P 〉0.05）. The expression of collagenⅣ and laminin in primary and secondary follicles were higher in PCOS group than control group （P〈 0.05）. The expression of collagen Ⅳ, laminin, and perlecan in pre-sinusoid and sinusoid follicles were obviously higher in PCOS group than control group （P〈 0.05）. [Conclusion] The increasing of the contents of basic membrane in PCOS developing follicles shows that the metabolism of ovarian extra cellular matrix is abnormal in women with PCOS.

关 键 词：多囊卵巢综合征 卵泡 基底膜 细胞外基质 

分 类 号：R711.75[医药卫生—妇产科学]