心力衰竭大鼠左心室β_3-肾上腺素能受体表达及其与心功能的关系  

Expression of β_3-adrenoceptors of Left Ventricle and Its Effect on Heart Function in Rats with Experimental Heart Failure

作　　者：邓义军[1] 伍卫[1] 杨蕙[1] 方昶[1] 黄至斌[1] 

机构地区：[1]中山大学附属第二医院心内科,广东广州510120

出　　处：《中山大学学报（医学科学版）》2006年第2期176-180,共5页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广州市科委基金资助项目(2004Z3-E0331)

摘　　要：【目的】观察实验性心力衰竭大鼠左心室β3-肾上腺素能受体(AR)表达水平的变化及β3-AR激动时对心功能的影响。【方法】雄性成年Wistar大鼠16只,随机分为心力衰竭组(n=8)及对照组(n=8)。结扎大鼠冠状动脉前降支致心肌梗死建立心力衰竭模型;使用RT-PCR法检测左心室β3-ARmRNA的表达;观察使用β3-AR激动剂BRL37344后,左室收缩末压(LVESP)、左室舒张末压(LVEDP)、左室最大压力上升和下降速率(dp/dtmax)等指标的改变。【结果】①与对照组相比,心力衰竭大鼠左心室β3-ARmRNA表达量(β3/β-actin的比值)增加(0.011vs0.028,P<0.05),所占比例[β3(/β1+β2+β3)]增加(1.2%vs5.4%,P<0.05);②静注BRL37344后,对照组LVESP、+dp/dtmax、-dp/dtmax分别下降12.2%、15.8%、11.5%,LVEDP上升9.8%;心力衰竭大鼠LVESP、+dp/dtmax、-dp/dtmax分别下降16.1%、21.7%、13.2%,LVEDP上升10.5%。【结论】心力衰竭大鼠左心室β3-ARmRNA表达明显增高,β3-AR激动时负性肌力作用明显增强。[Objective] To observe the expression of β3-adrenoceptors （β3-AR） of left ventricle and the effect on heart function by stimulating β3-AR in rats with experimental heart failure. [Methods] Rats were randomly distributed to heart failure group （n=8） and control group （n=8）. Heart failure models were built up by ligation of coronary artery in rats. The expression of β3-adrenoceptors mRNAs was detected with RT-PCR. The change of heart function was observed after administration of BRL37344 （β3-AR agonist） by measuring LVESP （left ventricular end-systolic pressure）, left ventricular end-diastolic volume CLVEDP）, the maximum pressure ascending rate of left ventricle （＋dp/ dtmax） and the maximum pressure descending rate of left ventricle （-dp/dtmax）.[Results] Both the expression of β3-AR nRNA （β3/β-actin） and the propertion [β3/（β1＋β2＋β3）] was increased in failure rats in comparison with control rats （0.028 vs 0.011 and 5.4% vs 1.2%）; The decreased percentages of LVESP, ＋dp/dtmax and -dp/dtmax, were 16.1%, 21.7%, and 13.2%, respectively after administration of BRL37344 in failure rats while the corresponding numbers were 12.2%, 15.8%, and 11.5% in control rats. [Conclusion] In comparison with non-failure rats, the expression of β3-AR mRNA of left ventricle was obviously increased and the negative inotropic function was obviously increased in failure groups when stimulating β3-AR.

关 键 词：Β3-肾上腺素能受体 心力衰竭 大鼠 

分 类 号：R54[医药卫生—心血管疾病]