卷蛾赤眼蜂体内共生菌Wolbachia对寄主产雌孤雌生殖行为的影响  

Effect of wasp symbionts Wolbachia on the thelytokous parthenogenesis of Trichogramma cacoeciae.

作　　者：项宇[1] 沈佐锐[1] 王伟晶[1] 黄大庄[2] 李瑞军[3] 

机构地区：[1]中国农业大学农学与生物技术学院,北京100094 [2]河北农业大学林学院,保定071000 [3]河北农业大学植保学院,保定071000

出　　处：《昆虫知识》2006年第2期219-222,共4页Entomological Knowledge

基　　金：国家自然科学基金项目(30471166);瑞典国际基金IFS资助项目(C/3257-1);博士点基金资助项目(20020019013)。

摘　　要：在30℃的条件下,连续饲养卷蛾赤眼蜂Trichogramma cacoeciaeMarchall10代,观察每代蜂的生物学特性,并进行Wolbachia的wsp基因的分子检测,以研究卷蛾赤眼蜂产雌孤雌生殖行为与其体内共生菌Wolbachia的关系。结果表明,高温下卷蛾赤眼蜂体内的Wolbachia被抑制,4代以后wsp基因的PCR检测结果变为阴性;同时赤眼蜂的产雌孤雌生殖行为的改变程度也随着世代的增加呈递增趋势。这显示卷蛾赤眼蜂的产雌孤雌生殖行为与其体内的共生菌Wolbachia具有一定程度的相关性。By molecular detection of Wolbachia wsp gene and observation of its host Trichogramma cacoeciae Marchall reproductive biology for every generation, we studied the effect of wasp symbionts Wolbachia on the thelytokous parthenogenesis of T. eacoeciae under the condition of 30℃ for 10 generations. After continuous heat treatment, the growth of Wolbachia was inhibited in the hody of T. cacoeciae and PCR product of Wolbaehia wsp gene turned to he negative after 4 generations. And the thelytokous parthenogenesis of T. cacoeciae increased with the generation. These results show that there exists some relationship between T. eaeoeeiae thelytokous parthenogenesis and its symbionts Wolbachia.

关 键 词：WOLBACHIA 产雌孤雌生殖 分子检测 高温 卷蛾赤眼蜂 

分 类 号：S476.3[农业科学—农业昆虫与害虫防治]