不同种源黄芩的RAPD分析  

RAPD analysis of Scutellaria baicalensis from different germplasms

作　　者：邵爱娟[1] 李欣[1] 黄璐琦[1] 林淑芳[1] 陈君[2] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]中国医学科学院中国协和医科大学药用植物研究所,北京100094

出　　处：《中国中药杂志》2006年第6期452-455,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(39930220)

摘　　要：目的:探讨黄芩不同种源的遗传多样性和亲缘关系,为黄芩育种提供依据。方法:采用14个10碱基随机引物,利用SPSS 10.0软件中的Within-groups linkage方法,对34个黄芩不同种源进行RAPD分析。结果:从115个引物中筛选出14个条带清晰、多态性明显并且重复性好的引物用于实验和统计分析,14个引物共扩出165个DNA片段,多态性DNA片段132个,占总扩增数的80.0%。34个黄芩种源可明显聚为A,B,C,D等4大类。结论:不同种源黄芩间具有丰富的遗传多样性;山东蒙阴3、山东蒙阴2、山东平邑种源间的遗传距离(0.315)较近,可考虑在黄芩系统选育中作为单一品种育种;黄芩种源间的遗传背景较为复杂,在遗传学上的分析结果与其外观形态大体上相似,但是与地理分布却没有一定的相关性,提示在黄芩良种选育过程中必须加强优良种子的选育和管理。Objective： To provide reference for breeding Scutellaria baicalensis from different germplasms,and to explore their genetic diversifies and different strains. Method： The RAPD method was applied to the study on S. baicalensis from different germplasms by use of 14 random primers about 10 bp, and SAPD-analysis of 34 S. baicalensis from different germplasms was carried out by using the method of withingoups-linkage in SPSS 10.0. Result： 14 among 115 primers were selected to amplify about 165 segments of DNA. Among them, 132 segments of DNA, 80.0% of the total, can represent genetic of diversities of S. baicalensis. According to RAPD analysis, 34 germplasms of S. baicalensis were classified into A, B, C and D categories. Conclusion： S. baicalensis from different germplasms shows abundant genetic diversities. Germplasms from Shandong like Mengyin 3, Menyin2 and Pingyi have close distance（0.315） in genetic hackground, which can be chosen for breeding of cultivated. Though genetic characters are similar in the morphology, the geological distribution of S. baicalensis and its morphology have not certain correlation. The complex genetic background of S. baicalensis indicate that the work of the selective breeding and management for breeding of S. baicalensis have to be strengthened.

关 键 词：黄芩 RAPD 选育 

分 类 号：S567.239[农业科学—中草药栽培]