3种姜黄色素干预ox-LDL促平滑肌细胞增殖与影响LDL-R表达的研究  

Effect of three different curcumin pigmens on the prdiferation of vascular smooth muscle cells by ox-LDL and the expression of LDL-R

作　　者：刘艳[1] 洪行球[1] 

机构地区：[1]浙江中医学院

出　　处：《中国中药杂志》2006年第6期500-503,共4页China Journal of Chinese Materia Medica

基　　金：浙江省自然科学基金项目(202015)

摘　　要：目的:研究3种不同结构的姜黄色素单体对牛主动脉平滑肌细胞(VSMC)增殖及牛VSMC低密度脂蛋白受体(LDL-R)表达的影响,解析构效关系。方法:用MTT比色法和流式细胞仪观察3种不同结构的姜黄色素单体对ox-LDL促牛VSMC增殖及对VSMC LDL-R表达的影响。结果:ox-LDL在2.5,5,10 mg.L-1时,对VSMC有明显的促增殖作用,P<0.05。姜黄素16.5,33,66μmol.L-1,一脱甲氧基姜黄素(简称一脱)33,66μmol.L-1和二脱甲氧基姜黄素(简称二脱)66μmol.L-1对10 mg.L-1ox-LDL培养的VSMC有明显的抑制作用,P<0.05。3种姜黄色素的抑制率为姜黄素>一脱>二脱。3种姜黄色素在66,33,16.5μmol.L-1时对VSMC LDL-R的表达均有明显的上调作用,其中姜黄素16.5μmol.L-1组与正常组比较,P<0.05,其他组与正常组比较P<0.01,上调作用二脱>一脱>姜黄素,经组间方差分析,P<0.01,与姜黄色素抑制牛VSMC增殖作用相反。结论:3种不同结构姜黄色素单体能明显抑制ox-LDL促牛VSMC增殖作用,上调牛VSMC LDL-R表达,从而有可能延缓动脉粥样硬化的发生发展过程。Objective： To investigate the anti-proliferation of three different structural curcumin pigmen monomers on the bovine VSMC stimulated by ox-LDL and the effect on the expression of LDL-R. Method： MTT and FCM were used to observe the anti-proliferation of three different on the bovine VSMC stimulated by ox-LDL and the effect on the expression of LDL-R. Result： ox-LDL（2.5,5,10 mg·L^-1 ） had obvious proliferative effect on VSMC, P 〈 0.05, curcmnin （ 16.5,33,66 μmol·L^-1 ）, demethoxycurcumin （33,66 μmol·L^-1 ） and bisdemethoxycurcumin （66 μmol·L^-1） had obvious inhibition effect on the proliferation of VSMC stimulated by 10 mg·L^-1 ox-LDL （ P 〈 0.05, curcumin 〉 demethoxycurcumin 〉 bisdemethoxycurcmnin）. Three curumnin （16.5,33,66 μmol·L^-1） could obviously activate the expression of LDLR of VSMC. Conclusion： The three different structural curcumin pigmen monomers can not only inhibit the proliferation of bovine VSMC obviously stimulated by ox-LDL, but also promote the expression of LDL-R on bovine VSMC, that may be the mechanism of delaying the development of arteriosclerosis.

关 键 词：姜黄素 一脱甲氧基姜黄素 二脱甲氧基姜黄素 平滑肌细胞 氧化低密度脂蛋白 低密度脂蛋 白受体 动脉粥样硬化 

分 类 号：R285.5[医药卫生—中药学]