β-氨基丁酸诱导黄瓜抗黄瓜炭疽病筛选体系的构建  

A screening system for β-aminobutyric acid induction of cucumber against colletotrichum lagenarium

作　　者：张永刚[1] 范志金[1] 刘秀峰[1] 苑建勋[1] 李正名[1] 

机构地区：[1]南开大学元素有机化学国家重点实验室,天津300071

出　　处：《农药》2006年第4期239-242,245,共5页Agrochemicals

基　　金：国家自然科学基金(批准号30270883);国家重点基础研究发展计划"973"项目(2003CB114402);农业部农药化学及农药使用技术重点实验室开放基金资助

摘　　要：为了给新型植物诱导抗病激活剂的创制提供基本的筛选平台,测定β-氨基丁酸对病原真菌的离体抑菌活性,探索了β-氨基丁酸诱导黄瓜植株对黄瓜炭疽病抗性的活体筛选方法,并探索了经β-氨基丁酸诱导和挑战接种前后黄瓜植株体内苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)、过氧化物酶(POD)和超氧化物歧化酶(SOD)活性的变化。结果表明,β-氨基丁酸无离体的抑菌活性,20mmol/L的β-氨基丁酸可以诱导黄瓜产生70%以上的抗病效果。生理生化测定结果表明,经BABA诱导处理黄瓜第一叶真叶后,提高新生真叶的PAL和POD的活性;电泳分析发现,β-氨基丁酸诱导接种后的黄瓜叶片产生了病程相关蛋白(PRP)。根据研究结果,提出了植物诱导抗病激活剂筛选的完整体系。in order to provide a basic screening platform for developing new plant disease resistance elicitors, in vitro fungal pathogen inhibition by β-aminobutyric acid （BABA） was determined. An in vivo screening method was investigated for determining BABA induction of cucumber anthracnose resistance in cucumber plants, along with changes in activities in cucumber plants of phenylalanine ammonia lyase （PAL）, polyphenyl oxidase （PPO）, peroxidase （POD）, and superoxide dismutase （SOD） before and after BABA induction and challenge. BABA showed no fungal inhibition activity in vitro. BABA at 20 mmol/L induced the production in cucumber of 〉70% disease resistance. Physiological and biochemical measurements indicated that BABA treatment of the first true leaf of cucumber caused an increase of PAL and POD activity in subsequently emerging leaves. Electrophoresis showed that cucumber leaves produced pathogenesis-related protein following induced inoculation with BABA. Based on the results of this research, a complete system is proposed for plant disease elicitors.

关 键 词：Β-氨基丁酸 系统获得抗性 苯丙氨酸解氨酶 病程相关蛋白 黄瓜炭疽病菌 

分 类 号：S436.421[农业科学—农业昆虫与害虫防治]