肺结核患者外周血白细胞中结核分枝杆菌DNA的检测  

Detection of myeobaeterium tuberculosis in peripheral blood from patients with pulmonary tuberculosis

作　　者：张吉旺[1] 彭晓[1] 朱俊芳[1] 

机构地区：[1]兰州医学院第一附属医院,730000

出　　处：《中华医学检验杂志》1996年第3期173-175,共3页

摘　　要：目的明确外周血白细胞中结核分枝杆菌 DNA 检测在肺结核诊断中的意义。方法应用聚合酶链反应技术,对95例肺结核患者外周血标本、84例肺结核患者痰标本进行了结核分枝杆菌DNA 检测。结果外周血、痰标本中,结核分枝杆菌 DNA 阳性率分别为85%和57%;对44例患者外周血、痰标本配对同时检测总阳性率可达91%。结论外周血白细胞中结核分枝杆菌 DNA 检测用于肺结核的诊断,可提高肺结核诊断的敏感性和准确性,明显优于痰标本,可作为肺结核诊断较可靠的指标。Objective To detect Mycobacterium tuberculosis in peripheral blood from patients with pulmonary tuberculosis.Methods Using polymerase chain reaction(PCR)technique,we amplified DNA of Mycobacterium tuberculosis in peripheral blood from 95 pulmonary tuberculosis patients.Re- sults The postivity rate of PCR in blood was 81/95(85%)which was significantly higher than that in sputum 48/84(57%).Combinative detection of blood and sputum in 44 patients showed that the sensi- tiveness and correctness of detection was increased,and the postivity rate was 91%.Conclusions This result indicated that the detection of DNA of Mycobacterium tuberculosis in peripheral blood was very useful for diagnosis of pulmonary tuberculosis.

关 键 词：肺结核 结核分枝杆菌 DNA 聚合酶链反应 

分 类 号：R521.04[医药卫生—内科学]