高胆固醇血症血清内胆固醇的光谱学研究  

Spectroscopy Research on Cholesterol in Hypercholesterolemia Serum

作　　者：兰秀风[1] 刘建刚[1] 刘莹[1] 骆晓森[1] 唐新国[2] 卢是钥[2] 倪晓武[1] 

机构地区：[1]南京理工大学信息物理与工程系,江苏南京210094 [2]中国人民解放军第二军医大学南京军医学院,江苏南京210099

出　　处：《光谱学与光谱分析》2006年第3期467-470,共4页Spectroscopy and Spectral Analysis

基　　金：高等学校优秀青年教师教学科研奖励计划项目(2003-2008)资助

摘　　要：应用光谱分析方法对高胆固醇血症血清中胆固醇的不同含量情形进行了研究,给出了正常人血清和高胆固醇血症血清的吸收光谱和荧光光谱,并分析了二者的谱线特征与差异。结果表明,高胆固醇血症血清的吸收光谱和荧光光谱不同于正常人血清的吸收光谱和荧光光谱,高胆固醇血症血清不仅是吸收率和荧光强度高于正常人血清的相应值,而且还有新的吸收峰和荧光峰出现。研究表明,通过比较待测血清的吸收光谱和荧光光谱可以初步判定血清中胆固醇的含量。这为发展一种新的血液中胆固醇含量的检测方法提供了实验依据。The cholesterol with different concentration in hypercholesterolemia serum was studied by the method of spectroscopy technology. The absorption and fluorescence spectra of normal human serum and hypercholesterolemia serum were obtained respectively; the spectral characteristic of each sample and the difference between two kinds of samples were discussed too. The results indicate that the absorption and fluorescence spectra of hypercholesterolemia serum are different from those of normal human serum. The absorptivity and the fluorescence intensity of hypercholesterolemia serum are both higher than those of normal human serum. Besides, there are new absorptive peaks and new fluorescence peaks in the spectrogram. Thus, the abnormalism of cholesterol in serum can be judged by comparing the absorption and fluorescence spectra. The researches in the present paper provide an experimental foundation for the diagnosis of cholesterol in blood.

关 键 词：高胆固醇血症 血清 吸收光谱 荧光光谱 胆固醇检测 

分 类 号：O433.4[机械工程—光学工程]