高效液相色谱法同时测定三黄片中的蒽醌类、黄酮类及生物碱类化合物  

Simultaneous determination of three kinds of components in Sanhuang tablets by high-performance liquid chromatography

作　　者：冯有龙[1] 余伯阳[1] 董小平[2] 

机构地区：[1]中国药科大学,江苏南京210009 [2]江苏省药品检验所,江苏南京210008

出　　处：《药学学报》2006年第3期285-288,共4页Acta Pharmaceutica Sinica

基　　金：教育部重点科研项目(2004)资助.

摘　　要：Aim To establish an assay method for the determination of three kinds of biologically active components,five compounds（emodin,chrysophanol,baicalin,wogonin and berberine hydrochloride） simultaneously in Sanhuang tablets.Methods HPLC was carried out,using a C18 column（150 mm×4.6 mm ID,5 μm） set at 30 ℃,acetonitrile-0.02 mol·L-1 acetic ammonium（adjusted pH to 3.50 with acetic acid glacial） as mobile phase（using gradient） with flowing rate 1.00 mL·min-1 and detected at 270 nm.Results The calibration curve of emodin was linear from（0.020 7） μg to 0.207 μg with r=0.999 9,the average recovery was 99.65% with RSD 1.25%.The calibration curve of chrysophanol was linear from 0.052 μg to 0.52 μg with r=（0.999 9）,and the average recovery was 100.36% with RSD 0.96%.The calibration curve of baicalin was linear from（0.250 5） μg to 2.505 μg with r=（0.999 8）,and the average recovery was 100.22% with RSD 1.29%.The calibration curve of wogonin was linear from 0.047 6 μg to 0.476 μg with r=（0.999 9）,and the average recovery was 98.97% with RSD 1.20%.The calibration curve of berberine hydrochloride was linear from（0.053 12） μg to 0.531 2 μg with r=（0.999 5）,and the average recovery was 96.02% with RSD 2.02%.The established method had also been used in the determination of the 5 compounds in 10 different batches of Sanhuang tablets.Conclusion This method was proved to be accurate and quick,and can be used for the quality control of the preparation all-around.Aim To establish an assay method for the determination of three kinds of biologically active components, five compounds （emodin, chrysophanol, baicalin, wogonin and berberine hydrochloride） simultaneously in Sanhuang tablets. Methods HPLC was carried out, using a C ls column （ 150 mm×4.6 mm ID, 5μm） set at 30℃, acetonitrile-0.02mol· L^-1 acetic ammonium （adjusted pH to 3.50 with acetic acid glacial） as mobile phase （using gradient） with flowing rate 1. 00 mL·min^-1 and detected at 270 nm. Results The calibration curve of emodin was linear from 0. 020 7μg to 0. 207 μg with r = 0.999 9, the average recovery was 99.65% with RSD 1.25%. The calibration curve of chrysophanol was linear from 0. 052μg to 0.52μg with r = 0. 999 9, and the average recovery was 100.36% with RSD 0.96%. The calibration curve of baicalin was linear from 0. 250 5μg to 2. 505μg with r = 0. 999 8, and the average recovery was 100. 22% with RSD 1.29%. The calibration curve of wogonin was linear from 0. 047 6 μg to 0. 476 μg with r = 0. 999 9, and the average recovery was 98.97% with RSD 1.20%. The calibration curve of berberine hydrochloride was linear from 0. 053 12μg to 0. 531 2μg with r = 0. 999 5, and the average recovery was 96.02% with RSD 2.02%. The established method had also been used in the determination of the 5 compounds in 10 different batches of Sanhuang tablets. Conclusion This method was proved to be accurate and quick, and can be used for the quality control of the preparation all-around.

关 键 词：高效液相色谱法 三黄片 大黄素 大黄酚 黄芩苷 汉黄芩素 盐酸小檗碱 

分 类 号：R917[医药卫生—药物分析学]