丝状固氮蓝藻柱胞鱼腥藻原生质球的培养再生  

REGENERATION OF SPHEROPLASTS IN A N_2- FIXED FILA MENTOUS BLUE GREEN ALGA ANABAENA CYLINDRICA

作　　者：郭厚良[1] 武汉大学生命科学学院 金传荫[1] 中国科学院水生生物研究所 宋文贞[1] 

机构地区：[1]武汉大学生命科学学院,武汉430072

出　　处：《Acta Botanica Sinica》1996年第8期637-640,共4页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家自然科学基金

摘　　要：丝状固氮蓝藻柱胞鱼腥藻 (Anabaena cylindrica)在含 0 .1mol/ L KCl的 Allen液体培养基中培养7～ 9d,90 %以上细胞转化为原生质球或半原生质球 ,对低渗敏感或抗低渗。此种材料经 0 .1%溶菌酶 ,在 2 8℃下处理 3～ 4h,细胞低渗破裂率约 10 0 % ,成为质量较高的原生质球。用 0 .15mol/ L Ca Cl2 液体无机盐培养基培养 ,原生质球再生和分裂。不同原生质球再生不同步 ,最快培养 3 d分裂。再生分裂的主要方式为均等分裂 ,但有不规则分裂和出芽方式 ,再生率在 2 5%以上。Over 90% of cells of Anabaena cylindrica growing in the medium containing 0 1 mol/L KCl for 7~9 d transformed into spheroplasts or semispheroplasts which were either sensitive or not sensitive to hypotonic condition. After treating the materials with 0 1% lysozyme at 28 ℃ for 3~4 h the transformed spheroplasts were almost 100% sensitive to the hypotonic condition. The spheroplasts then regenerated and divided through culture in the inorganic medium containing 0 15 mol/L CaCl 2 with a rate over 25%. The regeneration of different spheroplasts was not synchronous, the fastest division being after 3 d. Cell division was mainly equational but also irregular division or budding.

关 键 词：蓝藻 丝状固氮蓝藻 柱胞鱼腥藻 原生质球 再生 

分 类 号：Q949.220.5[生物学—植物学]