人羊膜上皮细胞在纤维蛋白支架上的生长情况及细胞生长因子对其生长的影响  

The effects of fibrin clot and cellular growth factor on the human amniotic epithelial cells.

作　　者：李玮[1] 漆洪波[2] 江庆霖[1] 周辉[1] 

机构地区：[1]成都市妇幼保健院妇产科,成都610031 [2]重庆医科大学附属第一医院妇产科

出　　处：《中华妇幼临床医学杂志（电子版）》2006年第2期74-77,共4页Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition)

基　　金：重庆市自然科学基金资助项目(03-8006)

摘　　要：目的人羊膜上皮细胞(human amniotie epithelial cells,HAEC)能否在体外构建的纤维蛋白支架上生长,以及表皮生长因子(epidema growth factor,EGF)、碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)和转化生长因子β1(transforming growth factor β1,TGF-β1)对HAEC增殖的影响。方法以纤维蛋白凝块为支架,构建HAEC生长模型,观察HAEC在纤维蛋白支架上生长情况(对照组),并加入细胞生长因子,包括EGF,bFGF,TGF-β1,观察细胞生长因子对HAEC生长的影响(研究组)。采用倒置显微镜、吉姆萨(Giemsa)染色和扫描电子显微镜观察HAEC的生长情况。采用脱氧核糖核苷酸末端转移酶介导的缺口末端标记(TUNEL)法分别检测纤维蛋白支架上HAEC凋亡情况。结果 HAEC在构建的纤维蛋白支架上生长良好。EGF组和bFGF组HAEC凋亡较对照组明显减少,差异有显著意义(P<0．05), TGF-β1组HAEC凋亡较对照组明显增加,差异有显著意义(P<0．05)。结论 HAEC能在构建的纤维蛋白支架上良好生长,EGF,bFGF可抑制HAEC凋亡,TGF-β1则促进HAEC凋亡。Objective To investigate whether human amniotic epithelial cells （HAEC）could be generate on the matrix of formulated fibrin clot in vitro and the effects of epidermal growth factor（EGF）, basic fibroblast growth factor（bFGF）and transforming growth factor β1, （TGF-β1）on the proliferation of HAEC. Methods The cultivated HAECs on the formulated fibrin clot were examined by phase contrast microseopy,Giemsa staining and scanning electron mieroscopy. We measured apoptosis of HAECs on the formulated fibrin clot with growth factor （EGF,bFGF,TGF-β1） by TUNEL. Results HAECs generate on the formulated fibrin clot in vitro. HAECs exposure to EGF or bFGP was associated with a lower rate of apoptosis than eontrol group （P〈0.05）, however, HAECs exposure to TGF-β1 showed opposite effect （P〈0.05）. Conclusion HAECs generate well on the formulated fibrin clot in vitro. HAECs exposure to EGF or bFGF was decreased apoptosis rate than control group, however, HAECs exposure to TGF-β1 was increased effect.

关 键 词：人羊膜上皮细胞 纤维蛋白 生长因子 

分 类 号：R714[医药卫生—妇产科学]