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作 者:易念[1] 张纯[1] 王云[1] 刘少军[1] 刘筠[1]
机构地区:[1]教育部蛋白质化学和鱼类发育生物学重点实验室湖南师范大学生命科学学院,中国长沙410081
出 处:《湖南师范大学自然科学学报》2006年第1期87-91,104,共6页Journal of Natural Science of Hunan Normal University
基 金:国家自然科学基金重点项目(30330480);国家"973"项目(No.2001CB109006);教育部高等学校博士学科专项科研基金资助项目(200405422001)
摘 要:以异源四倍体鲫鲤为父本,二倍体金鱼为母本通过倍间杂交形成三倍体鲫鱼.对三倍体鲫鱼早期胚胎发育过程进行了观察.三倍体鲫鱼成熟卵子呈黄色,圆形,有粘性,卵径为1.3—1.4mm.水温为(20±1)℃情况下,卵子受精后1h,卵裂开始.受精后在-3h时间段内进行卵裂.受精后3.50h逐渐进入早、中、晚囊胚期,受精后10h进入原肠期,受精后15.50h进入神经胚期,19.0h后器官开始形成.受精后63—75h,仔鱼孵化出膜.另外,对三倍体鲫鱼受精卵进行切片观察,结果表明:三倍体囊胚期细胞分裂旺盛,原肠期细胞表现出正常的细胞分裂、细胞变形和迁移行为.结合三倍体鲫鱼胚胎外观发育观察,说明三倍体鱼具有正常的早期胚胎发育过程,为三倍体鱼的大规模生产提供了胚胎发育的生物学依据.The early embryonic development of triploid crucian carp which was generated by mating the males of the allotetraploid hybrids of red crucian carp( Carassius auratus red var. ) ( ♂ ) × common carp ( Cyprinus carpio L. ) ( ♀ ) with the females of the goldfish( Carassius auratus red var. ) is described. The mature eggs of triploid crucian carp are yellow, globose and sticky. The average diameter of these eggs is 1.3 - 1.4 mm. At the water temperature of (20 ± 1) %, the fertilized eggs begin the first cleavage 1 h after fertilization and reach morula stage (3 h), blastula stage (3. 50 h), gastrula stage (10 h), neural stage (15.50 h), organogenetic stage (19.50 h) gradually; and were hatched out 63 - 75 h after fertilization. In addition, the cytological behavior in fertilized eggs of triploid Crucain Carp coming from Allotetraploid Hybrids ( ♂ ) × Goldfish( ♀ ) is observed through tissue section. The results indicate that the embryonic cells have normal and frequent mitotic behavior during blastula stage and moved normally during gastrula stage.
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