龙眼果皮过氧化物酶的分离纯化  

Purification of peroxidase from the pericarp of longan

作　　者：林琳[1] 郭志雄[1] 潘东明[1] 

机构地区：[1]福建农林大学园艺学院

出　　处：《福建农林大学学报（自然科学版）》2006年第2期157-160,共4页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家"948"计划资助项目(991029);福建省科技厅资助项目(2001I008)

摘　　要：以福眼龙眼(D im ocarpus longanaLour.cv.Fuyan)果皮为材料,经硫酸铵盐析分级,以及Stream line Phenyl、DEAE-Cel-lu lose、Phenyl Sepharose H igh Perform ance、Superdex 200 prep grade等柱层析分离,得到由分子质量约45.6 ku的单一亚基组成的4个POD组分,其分子质量分别为290.1、232.1、188.5和170.7 ku.Using pericarp of mature longan （Dimocarpus longana Lout. cv. Fuyan. ） as material, after fraction with 40% saturation of ammonium sulfate, peroxidase （POD） was purified by Streamline Phenyl, DEAE-Cellulose, Phenyl Sepharose High Performance and Superdex 200 prep grade, respectively. The result showed that 4 PODs composing single subunit （45.6 ku） were obtained, which molecular weights were 290.1,232.1,188.5 and 170.7 ku, respectively.

关 键 词：龙眼 过氧化物酶(POD) 分离纯化 

分 类 号：S667.2[农业科学—果树学] Q814.1[农业科学—园艺学]