亚低温和zVAD-fmk联合治疗新生鼠缺氧缺血性脑损伤  

Neuroprotective effects of hypothermia and Caspase inhibitor on hypoxic-ischemic brain damage in neonatal rats

作　　者：柴斌英[1] 刘家浩[1] 王海英[1] 赵玉祥[1] 黄秋玲[1] 唐洪丽[1] 

机构地区：[1]东南大学附属中大医院儿科,南京210009

出　　处：《江苏医药》2006年第4期340-342,共3页Jiangsu Medical Journal

基　　金：东南大学医学研究基金(4922003YJ15)

摘　　要：目的探讨亚低温与zVADfmk对缺氧缺血性脑损伤的保护作用。方法72只缺氧缺血(HI)模型鼠随机分6组(每组12只):亚低温(29℃,A)组,zVADfmk治疗(B)组,亚低温+zVADfmk联合治疗(C)组,常温恢复(D)组和安慰剂(E)组、假手术对照(F)组。观察不同干预方法对大鼠脑水肿、皮层和海马Caspase3表达、海马神经元死亡率和细胞凋亡百分率的影响。结果A、B、C组脑水肿程度、Caspase3表达、海马神经元死亡率和细胞凋亡百分率均显著低于D组,C组降低更明显。E组和F组各指标相差无显著意义。结论亚低温、zVADfmk、亚低温与zVADfmk联合治疗均可显著减轻HI后的脑损伤,联合治疗对神经保护效果更佳。Objective To investigate the protective effects of mild hypothermia and/or Caspase inhibitor（zVADfmk：benzyloxycarbonybVal-Ala-Asp-fluoromethyl ketone） on hypoxic-ischemic brain damage（HIBD）. Methods The model rats （subjected to unilateral left carotereal artery ligation and given 8% oxygen for 2 h） were randomly allocated to HI-29℃ group（A）, zVAD（B）, HI-29℃ ＋ zVAD （C）, HI-37℃ （D） and placebo group（E）. The control group（F）was shamoperated animals. Changes of brain edema, expression of Caspase-3 in the cortex and hippocampus, percentage of hippocampal dead neurons（HDNP） and apoptotie cells in the hippocampus were investigated. Results Brain edema, expression of Caspase-3 in the cortex and hippocampus, HDNP and apoptotic cells in the hippocampus were significantly decreased in group B, C and D compared with those in group A. The most obvious attenuation was observed in group D. No significant difference existed between group E and group F. Conclusion Mild hypothermia and/or zVAD-fmk have protective effects on HIBD, especially when mild hypothermia and zVAD-fmk are used simultaneously.

关 键 词：低温 CASPASE抑制剂 脑缺氧 脑缺血 

分 类 号：R651.2[医药卫生—外科学] R722.12[医药卫生—临床医学]