celB基因标记法研究酸性土花生接种及施钼效果  

Study the effects of Mo and inoculation of Bradyrhizobium in acid purple soil by celB gene maker

作　　者：于景丽[1] 张小平[1] 李登煜[1] 陈强[1] 王可美[1] 

机构地区：[1]四川农业大学资源与环境学院微生物系

出　　处：《植物营养与肥料学报》2006年第2期250-253,共4页Journal of Plant Nutrition and Fertilizers

基　　金：国家自然科学基金项目(39970029);"973"项目(2001CB108905)资助

摘　　要：以缺钼酸性紫色土为供试土壤做盆栽试验,选用celB标记的三株慢生型花生根瘤菌celB3-5、celB3-7、celB4-5接种天府9号花生。通过标记根瘤菌形成的根瘤能与检测试剂产生颜色反应的特征,检测施钼及施不同浓度的钼对花生-根瘤菌有效性和竞争性的影响。结果表明,缺钼酸性紫色土上单施钼、单接种、接种配合施钼均能促进花生与根瘤菌的共生固氮效应和竞争结瘤能力,但接种配合施钼的效果最好,单接种的效果次之,单施钼的效果差。单施钼时,0.4%的钼酸铵拌种效果好,接种根瘤菌时,0.2%的效果好。供试菌株中celB4-5的有效性和竞争性最强,celB3-7次之,celB3-5差。In this research, an acid purple soil deficient in Mo was used for pot experiment. Three strains of celB-labeled Bradyrhizobium celB 3-5, celB 3-7, celB 4-5 were selected to inoculate Tianfu 9 peanut. Root nodule formed by gene marker strains would have color reaction with the test reagent. This characteristic can be used to detect effect of Mo and its application rate on the effectiveness and competitiveness of the peanut-Bradyrhizobium symbiosis. Results show that in the acid purple soil Mo deficit, single application of Mo, single inoculation of Bradyrhizobium or combination of the two all could promote symbiotic nitrogen-fixation and competitive ability of peanut- Bradyrhizobium in nodulation, among which the effect of combination was the best, followed by inoculation and single Mo application. The effect of application of 0.4% NH4MoO3 solution was more pronounced than that of 0.2% NH4MoO3 solution when applied alone, whereas the effect of the former was less pronounced than that of the latter when combined with inoculation. In terms of effectiveness and competitiveness, Strain celB 4-5 took the best and followed with celB 3-7 and celB 3-5.

关 键 词：缺钼 celB基因 花生根瘤菌 有效性 竞争性 

分 类 号：S565.2[农业科学—作物学] S792.260.2