鸽源冠状病毒PSH株纤突蛋白基因的遗传变异分析  

Genetic variation in spike glycoprotein gene of coronavirus isolated from pigeon

作　　者：钱华冬[1] 吴祖立[1] 华修国[1] 朱建国[1] 

机构地区：[1]上海交通大学农业与生物学院动物科学系,上海201101

出　　处：《中国兽医科学》2006年第3期199-202,共4页Chinese Veterinary Science

基　　金：上海交通大学-昂立抗击"非典"科技基金项目(SARS03-03)

摘　　要：对鸽源冠状病毒PSH株纤突蛋白基因进行了RT—PCR扩增、克隆和测序。结果表明，PSH株S基因由3504个核苷酸组成，编码1条由1167个氨基酸残基组成的多肽。S基因编码产物裂解后形成的S1和S2亚单位分别由541和626个氨基酸残基组成。PSH株S蛋白切割识别位点为精氨酸-精氨酸-苯丙氨酸-精氨酸-精氨酸（RRFRR），与多数鸡传染性支气管炎病毒（IBV）切割识别位点相似（RRF／SRR）。与GenBank中其他冠状病毒毒株S基因的推导氨基酸序列相比较，PSH株与IBV参考毒株的同源性为79．3％～99．6％，而与其他冠状病毒包括火鸡蓝冠病病毒和SARS病毒的同源性均小于37．8％。表明，鸽源冠状病毒PSH株属于第3抗原群冠状病毒，且与IBV亲缘关系较近。Spike glycoprotein gene of PSH strain of coronavirus of pigeon origin was amplified by RTPCR, cloned, sequenced and compared with the published spike genes sequences of coronaviruses from GenBank. Results showed that the S gene of PSH strain was 3 504 nucleotides in length, encoding a polypeptide of 1 167 amino acids. The cleavage site sequence of the S glycoprotein of the PSH strain contained five consecutive basic amino acids, namely, Arg-Arg-Phe-Arg-Arg（RRFRR）, which was similar to the majority of avian infectious bronchitis virus （IBV） strains. The deduced S1 and S2 proteins were composed of 541 and 626 amino acids, respectively after S protein was cleaved. A high degree of sequence identity （79.30%-99.60%） was observed in the S glycoprotein sequences between the PSH strain and IBV, but only low similarity （〈37.8%） was observed with turkey coronavirus （TCoV） and other mammalian coronaviruses. Based on these findings, the virus was tentatively identified as a member of group 3 coronaviruses which has closer relationship with the IBV strains.

关 键 词：冠状病毒 鸽 纤突蛋白 遗传变异 

分 类 号：S852.659[农业科学—基础兽医学]