人16型乳头瘤病毒“假病毒”的制备  

In Vitro Construction of Pseudovirions of Human Papillomavirus Type 16

作　　者：秦焱[1] 崔红莲[1] 胡美浩[1] 

机构地区：[1]北京大学生命科学学院,北京100871

出　　处：《北京大学学报（自然科学版）》2006年第2期180-183,共4页Acta Scientiarum Naturalium Universitatis Pekinensis

摘　　要：利用杆状病毒表达体系,在sf9细胞中表达人16型乳头瘤病毒(Human Papillomavirus type16,HPV16)的晚期表达蛋白L1,通过超速离心的方法得到HPV16病毒样颗粒(virus like particles,VLPs)。VLPs经过DTT和EDTA处理解聚成L1蛋白,将构建好的真核表达载体E6E7-pcDNA3.1+与解聚的L1蛋白混合,透析除去DTT和EDTA并逐渐升高Ca2+的浓度,解聚的L1能够重新聚集成VLPs,其中一部分VLPs包裹了真核表达载体,和天然的人乳头瘤病毒的构成很相似,作者称之为“假病毒”。The L1 major capsid protein of human papillomavirus type 16 was expressed in sf9 insect cells by recombinant baculoviruses and self-assembled into virus-like particles （VLPs）. The VLPs were harvested by CsCl ultracentrifugation and were disassemhled with DTT and EDTA. A reassembled process was performed by removing DTT and EDTA in the presence of E6E% pcDNA3.1 ＋ plasmid and Ca^2＋ . The novel VLPs, in which the plasmid was packed, were named HPVl6 pseudovirus.

关 键 词：人16型乳头瘤病毒 杆状病毒表达系统 假病毒 

分 类 号：R373[医药卫生—病原生物学]