壳聚糖固定化云芝漆酶的制备及特性  

Preparation and Characteristics of Immobilized Laccase from Coriolus versicolor on Chitosan

作　　者：陈辉[1] 张剑波[1] 王维敬[1] 杨宇翔[2] 

机构地区：[1]北京大学环境学院环境科学系,北京100871 [2]华东理工大学化学与制药学院,上海200237

出　　处：《北京大学学报（自然科学版）》2006年第2期254-258,共5页Acta Scientiarum Naturalium Universitatis Pekinensis

基　　金：国家自然科学基金项目(20577010);中意合作UNDP项目(CPR/01/R51/A/CC/31)

摘　　要：以壳聚糖为载体,戊二醛为交联剂制备固定化漆酶,测定了不同戊二醛浓度、交联时间、给酶量以及固定化时间对固定化漆酶活性的影响。结果表明,固定化反应的最佳条件为戊二醛浓度5%,交联时间8h,给酶量20mg/g载体,固定化时间6h,在此条件下制备的固定化漆酶酶活性保持率为52.2%。此固定化漆酶具有最大活性时的溶液pH值在4.5,相比于自由漆酶的最佳pH值5.0,其向酸性偏移;与自由漆酶相比,固定化酶有很好的稳定性和可重复使用性。Laccase has been immobilized on chitosan with glutaraldehyde as cmsslinking agent. The effects on the activity of immobilized laccase, such as concentration of glutaraldehyde, crosslinking time, laccase dosage and reaction time were studied. The results showed the optimal conditions of immobilization： the optimal glutaraldehyde concentration was 5%, the optimal cmsslinking time was 8 h, the optimal laccase dosage was 20 mg/g carrier, and the optimal reaction time was 6 h. On this condition, the recovery of enzyme activity was 52.2%. The resuhs indicated that the optimal pH for immobilized laccase was around 4.5, which is lower than the best pH 5.0 for free enzyme. Compared with the free enzyme, its stability and repetition were considerably improved.

关 键 词：活性 保持率 最佳pH值 稳定性 

分 类 号：TQ28[化学工程—有机化工]