助溶剂对发光菌生物毒性测试的影响  

Influence of cosolvents on Photobacterium phosphoreum toxicity test

作　　者：董玉瑛[1] 雷炳莉[2] 马静[1] 王芳[1] 

机构地区：[1]大连民族学院环境工程系国家民委生物化学工程重点实验室,辽宁大连116600 [2]河南师范大学化学与环境科学学院河南省环境污染控制重点实验室,河南新乡453002

出　　处：《化工学报》2006年第3期636-639,共4页CIESC Journal

基　　金：教育部留学回国人员科研启动基金项目(21841013)~~

摘　　要：There is little information about the influence of organic solvents for low water-solubility organic compounds on Photobacterium phosphoreum toxicity test,though it appears likely that much of data were obtained by using solutions prepared with cosolvents or surfactants.In this study the influence of five stock solvents,including dimethyl sulphoxide（DMSO）,acetone,aether,ethanol,tetrahydrofuran,on Photobacterium phosphoreum toxicity test was measured and different effective concentration values were obtained.DMSO was selected as the most suitable solvent for organic compounds with low water-solubility according to its good characteristics and less toxicity effect on Photobacterium phospherum.Chlorobenzene and nitrobenzene with proper solubility were selected to verify the effect of DMSO on Photobacterium phosphoreum toxicity.It was demonstrated that DMSO(concentration no higher than 0.20 mol·L-1) had little effect on the toxicity of organic chemicals with relative error no more than 10%.The successful application of DMSO as cosolvent provided a good example and a good idea to improve traditional acute toxicity test of Photobacterium phosphoreum,and broadened the application to identifying the toxicity of low water-solubility organic compounds.There is little information about the influence of organic solvents for low water-solubility organic compounds on Photobacterium phosphoreum toxicity test, though it appears likely that much of data were obtained by using solutions prepared with cosolvents or surfactants. In this study the influence of five stock solvents, including dimethyl sulphoxide （DMSO）, acetone, aether, ethanol, tetrahydrofuran, on Photobacterium phosphoreum toxicity test was measured and different effective concentration values were obtained. DMSO was selected as the most suitable solvent for organic compounds with low water-solubility according to its good characteristics and less toxicity effect on Photobacterium phospherum. Chlorobenzene and nitrobenzene with proper solubility were selected to verify the effect of DMSO on Photobacterium phosphoreum toxicity. It was demonstrated that DMSO （concentration no higher than 0.20 mol·L^-1） had little effect on the toxicity of organic chemicals with relative error no more than 10%. The successful application of DMSO as cosolvent provided a good example and a good idea to improve traditional acute toxicity test of Photobacterium phosphoreum, and broadened the application to identifying the toxicity of low water-solubility organic compounds.

关 键 词：发光菌 助溶剂 二甲亚砜 毒性影响 

分 类 号：X503.2[环境科学与工程—环境工程]