哇巴因和高钾对乙酰胆碱致家兔肺动脉内皮依赖性超极化的作用  

Effects of oubaine and high potassium on acetylcholine-induced endothelium-dependent hyperpolarization in rabbit pulmonary artery

作　　者：崔毅[1] 赵德化[1] 

机构地区：[1]西安第四军医大学基础部药理教研室

出　　处：《中国药理学通报》1996年第1期50-52,共3页Chinese Pharmacological Bulletin

摘　　要：采用标准微电极技术观察了哇巴因和高钾对乙酰胆碱（ａｃｅｔｙｌｃｈｏｌｉｎｅ，Ａｃｈ）致家兔肺动脉内皮依赖性超极化的作用。结果；Ａｃｈ１０μｍｏｌ·Ｌ－１可使内皮完整的肺动脉膜电位产生明显的超极化。该作用在去除内皮或预先用阿托品阻断Ａｃｈ受体的条件下消失。哇巴因可抑制无钾／复钾所致的膜电位超极化，但不影响Ａｃｈ所致的内皮依赖性超极化。高钾则可取消Ａｃｈ的内皮依赖性超极化。提示：Ａｃｈ引起的家兔肺动脉内皮依赖性超极化可能由细胞膜上钾通道开放所致，而和Ｎａ／Ｋ泵无关。Effects of oubaine and high potassium on acetylcholine(Ach)induced endothelium-dependent hyperpolarization in rabbit pulmonary artery were studied by using intracellular microelectroide techniques.Ach induced significant hyperpolarization of membrane potential in pulmonary artery with endothelium.The Ach-induced hyperpolarization was disappeared when endothelium was removed,and blocked by atropine.The hyperpolarization induced by K+after incubation with K+-free solution was inhibited by oubaine, whereas the Ach-induced hyperpolarization was not affected by oubaine.The high K+ solution could inhibit the Ach-induced hyperpolarization. The results suggest that the potassium Channels not the Na/K pump might contribute to the Ach-induced hyperpolarization in rabbit pulmonary artery.

关 键 词：哇巴因 钾离子 乙酰胆碱 内皮 超极化 肺动脉 

分 类 号：R972[医药卫生—药品]