气态甲醛致小白鼠肝脏DNA-DNA交联的研究  

Study on DNA-DNA crosslinks induced by air formaldehyde in mice liver

作　　者：何胡军[1] 鲁志松[2] 刘英帅[2] 彭光银[1] 丁书茂[1] 杨旭[1] 

机构地区：[1]华中师范大学生命科学学院,武汉430079 [2]南洋理工大学化学与生物医学工程学院,新加坡639798

出　　处：《环境科学学报》2006年第3期501-504,共4页Acta Scientiae Circumstantiae

基　　金：国家自然基金项目(NO.30570799)~~

摘　　要：为了探讨气态甲醛致生物体内DNADNA交联效应,进一步评价甲醛的遗传毒性作用,以昆明纯系小鼠为实验材料,进行了72h动态吸入式连续染毒,采用荧光检测法检测甲醛染毒后小鼠肝细胞DNADNA交联形成的效应.实验结果表明,0.5mg·m-3的气态甲醛能引起明显的DNADNA交联(p<0.05),较高浓度的甲醛(1.0mg·m-3、3.0mg·m-3,p<0.01)可以产生极为明显的DNADNA交联作用.以上实验结果显示了0.5mg·m-3的气态甲醛就能致生物体内DNADNA交联效应,且随着浓度的升高DNADNA交联率越高.In order to explore the effect of DNA-DNA crosslinks and estimate the genotoxicity induced by formaldehyde the experiment objects, which were exposed to the air formaldehyde in 72 hours. This experiment applied fluorescence this study used Kunming mice as t to detect DNA-DNA crosslinks formation effect of the liver cells of the mice after the exposure to air formaldehyde. Results show that 0.5 mg·m^-3 air formaldehyde can induce DNA-DNA crosslinks formation in vivo （p 〈0.05）, and in a dose-dependent manner at higher concentrations （ 1.0 mg·m^-3, 3.0 mg·m^-3 ,p 〈 0. 01 ）. Air formaldehyde exposure, even as low as 0.5 mg·m^-3 , induced DNA-DNA crosslinks in mice liver easily, and the effect level is taken in a dose-dependent manner within 3.0 mg·m^-3 range.

关 键 词：甲醛 DNA-DNA交联 遗传毒性 体内 

分 类 号：R994.6[医药卫生—毒理学]