商陆多糖I对小鼠淋巴细胞DNA多聚酶α活性的影响  

Effect of Phytolacca acinosa polysaccharides I on DNA polymeraseα activity in murine splenocytes

作　　者：王洪斌[1] 王劲[1] 郑钦岳[1] 李铁军[1] 李书桐[1] 陈海生[1] 

机构地区：[1]第二军医大学药学院药理学教研室,第二军医大学药学系,西安空军医院,第二军医大学药学院植物化学教研室

出　　处：《第二军医大学学报》1996年第2期150-153,共4页Academic Journal of Second Military Medical University

摘　　要：本实验在建立ＤＮＡ多聚酶α活性测定方法的基础上观察了商陆多糖Ｉ（ＰＡＰ－Ｉ）对小鼠淋巴细胞增殖及淋巴细胞ＤＮＡ多聚酶α活性的影响。采用［３Ｈ］－ＴｄＲ掺入法和模板活化法（［３Ｈ］－ｄＴＴＰ掺入法）分别检测ＰＡＰ－Ｉ给药组及对照组小鼠淋巴细胞增殖能力和淋巴细胞ＤＮＡ多聚酶α活性。体外实验发现ＰＡＰ－Ｉ显著增强ＣｏｎＡ诱导的小鼠脾淋巴细胞增殖及其ＤＮＡ多聚酶α的活性；小鼠腹腔注射ＰＡＰ－Ｉ（每周１次）对脾淋巴细胞ＤＮＡ多聚酶α活性基本上无影响，但加入ＣｏｎＡ（５μｇ／ｍｌ）刺激后，ＰＡＰ－Ｉ１０ｍｇ／ｋｇ剂量组ＤＮＡ多聚酶α活性显著增强。结果提示ＰＡＰ－Ｉ增强ＤＮＡ多聚酶α活性可能是促进脾淋巴细胞增殖、增强免疫功能的机制之一。The purpose of the present study was to investigate the effect of Phytolacca acinosa polysaccharides(PAP-I) on DNA polymerase a activity of murine splenocytes and their proliferation.The activity of DNA polymerase a was measured by the method of [3H]-dTTP uptake by template DNA which was heated at 100℃for 5 min.The proliferation ability of murine splenocytes was tested by [3H]-TdR uptake by the splenocytes.The DNA polymerase a activity was found to be augmented by PAP-I at the dosage of 200μg/ml.In the same time,DNA synthesis of murine splenocytes was also increased. The DNA polymerase a activity from murine splencoytes,which were treated with ip PAP-I 5,10,50 mg/kg,was also measured in the present study.The results showed that PAP-I,10 mg/kg ip could significantly augment DNA polymerase a activity from murine splenocytes when the murine splenocytes were treated with Con A 5 μg/ml.DNA synthesis of murine splenocytes was also augmented by administration of PAP-I,10 mg/kg ip.These data indicate that enhancing effect of PAP-I on lymphocyte proliferation might be related with its augmenting effect on DNA polymerase a activity from murine splenocytes.

关 键 词：商多糖 DNA 多聚酶α 淋巴细胞 中药 

分 类 号：R286.95[医药卫生—中药学]