β－内酰胺酶介导的阴沟杆菌对β－内酰胺类抗生素耐药机制的研究  

Study on β-lactamase-mediated resistant mechanism of Enterobacter cloacae to β-lactam antibiotics

作　　者：黎世能[1] 王浴生[1] 雷军[1] 

机构地区：[1]华西医科大学基础药理室,成都中医学院附属医院

出　　处：《中国抗生素杂志》1996年第2期121-123,152,共4页Chinese Journal of Antibiotics

摘　　要：选用β－内酰胺酶量明显增加的耐药阴沟杆菌，用透析方法分析β－内酰胺酶与对酶稳定抗生素氨曲南和对酶不稳定抗生素头孢孟多相互作用过程，以研究酶介导的细菌耐药机制。结果表明，产酶株对头孢孟多的耐药以酶对药物的水解为主；对氨曲南的耐药依据β－内酰胺酶与氨曲南的不同作用过程而不同。可以是酶对药物“牵制”和缓慢水解同时并存，也可能是其它机制。Resistant mechanism of Enterobacter cloacae producing large amount of β-lactamase was studied by dialyzing the β-lactamase against a non-substrate antibiotic,aztreonam(AZ)and a substrate antibiotic,cefamandole(CMD)respectively,The activity of β-lactamase in dialysis bag and the concentration of antibiotic in the outer compartment were mesured by UV and microbial assay concomitantly.Results showed that the resistant mechanism of the organism to CMD was hydrolysis of the drug,while the resistant mechanism to AZ was depended upon the interaction between AZ and the β-lactamase,It could be‘ontained’and/or slowly hydrolyzed of AZ by β-lactamase or other mechanisms.

关 键 词：氨曲南 头孢孟多 Β-内酰胺酶 阴沟杆菌 抗药性 

分 类 号：R978.11[医药卫生—药品] R378[医药卫生—药学]