反相高效液相色谱法分析测定头孢克洛  

Determination of cefaclor by reversed-phase HPLC

作　　者：张哲峰[1] 梁贵键[1] 鹿颐[1] 刘炜[1] 刘远晓 李现杰[1] 

机构地区：[1]河北省药品检验所,北京空军总医院

出　　处：《中国抗生素杂志》1996年第3期188-190,209,共4页Chinese Journal of Antibiotics

基　　金：国家自然科学基金

摘　　要：以磷酸盐缓冲液（ｐＨ３．４）－甲醇（７１：２９）为流动相在Ｃ１８往上分离测定头孢克洛（ＣＣＬ），检测波长２５４ｎｍ，乙酰苯胺作内标。ＣＣＬ进样量在０．４～２．４μｇ间线性关系良好，加样回收率９９．８％，重复进样的ＲＳＤ为０．３６％（ｎ＝１０）．ＣＣＬ在Ｃ１８柱上的色谱行为表明在ｐＨ３．５～６．０环境中，其分子中的氮基及羧基均可离子化。其水溶液在ｐＨ２～５时较稳定，大于ｐＨ６则易降解。An HPLC method was described for the determination of cefaclor.The chromatographic conditions were column:C18;mobile phase:pH3.4 phosphate buffermethanol(71:29):detected at 254nm with acetanilide as internal standard.The standard curve was rectilinear within the range of 0.4￣2.4μg. The recovery of cefaclor was 99.8%.The relative standard deviation for repetition was 0.36%(n=10).The chromatographic behavior of cefaclor on a C18 column proved that its amine and carboxyl groups were all be ionized when.pH was 3.5￣6.0. The solution of cefaclor was found to be stable when pH was 2～5, and degradable when pH was more than 6.

关 键 词：头孢克洛 高效液相色谱 稳定性 头孢菌素 

分 类 号：R978.11[医药卫生—药品] R927.2[医药卫生—药学]