应用聚合酶链式反应和原位杂交方法检测胎儿组织中人微小病毒B19核酸序列  

PCR and in situ hybridization for the detection of human parvovirusB19 nucleic acid sequences in fetal tissues

作　　者：曹虹[1] 郭辉玉[1] 卓礼梅[1] 张文炳[1] 

机构地区：[1]第一军医大学微生物学教研室,中山医科大学微生物学教研室

出　　处：《第一军医大学学报》1996年第1期17-19,共3页Journal of First Military Medical University

基　　金：美国中华医学基金

摘　　要：用聚合酶链式反应（ＰＣＲ）法检测了７例胎儿的１２份组织标本，在４份脑组织和１份脾组织中扩增出７００ｂｐ片段，经酶切鉴定证明其为特异性片段。用地高辛标记的人微小病毒Ｂ１９（ＰＶＢ１９）ＤＮＡ探针对ＰＣＲ检测阳性标本进行原位杂交，在２份脑组织和１份脾组织中检出了ＰＶＢ１９ＤＮＡ阳性颗粒，ＰＣＲ和原位杂交方法结合应用可为胎儿ＰＶＢ１９感染的病原学诊断和致病机理的研究提供一套持异、敏感和准确的实验方法。Attempts were made to detect human parvovirus B19 DNA by PCR and in situ hybridization in fetal tissues from 7 fetuses,Parvovirus B19 DNA:sequences were detected by PCR and positive results wereobtained in 4 samples of brain tissue and 1 of spleen tissue from these fetuses,In situ hybridization withDigoxigenin- labelled probe for human parvovirus B19 was performed on formalin-fixed paraffin-embeddedtissues from these fetuses,Parvovirus;DNA could be detected precisely and localized mainly in the nuclei ofImmature hematopoetic cells within fetal blood vessels, PCR and in situ bybridization were established to provide specific,sensitive and accurate methods for pathologic diagbosis and pathogenecity study of par-vovirus B19、

关 键 词：人微小病毒B19 原位杂交 胎儿 聚合酶链反应 

分 类 号：R714.5[医药卫生—妇产科学] R373[医药卫生—临床医学]