拟除虫菊酯类农药的非对映和对映异构体分离  

Separation of the diastereomers and enantiomers isomers of synthetic pyrethroids.

作　　者：塔娜[1] 李想[1] 冯建芳[1] 刘廷凤[1] 孙成[1] 

机构地区：[1]南京大学环境学院,污染控制与资源化国家重点实验室,江苏南京210093

出　　处：《中国环境科学》2006年第1期87-90,共4页China Environmental Science

基　　金：中国科学院南京土壤所土壤圈物质循环开放实验室资金支持

摘　　要：正己烷，异丙醇为流动相的正相体系中，使用Supelcosil LC-CN氰基柱完全分离了氯氰等多种拟除虫菊酯农药的非对映异构体；使用Supelcosil LC-CN保护柱串联chiralcel OD-H手性柱以及使用Supelcosil LC—CN氰基柱串联chiralcel OD—H手性柱实现了氯氰等多种拟除虫菊酯类农药的对映异构体分离．流动相中异丙醇的比例对保留时间和分离度的影响较大，随着异丙醇比例的减少，保留时间和分离度逐渐增加：在0～30℃间，柱温对手性识别能力无明显的影响；但对保留时间影响明显，随着柱温的增加，保留时间缩短．Separation of the diastereomers and enantiomers of synthetic pyrethroids was performed by high performance liquid gromatography （HPLC） with different columns in normal phase. The Supelcosil LC-CN column was applied to separate the diastereomers of commonly used pyrethroids such as cypermethrin etc.; the Supelcosil LC-CN precolumn and the Chiralcel OD-H column were chained to separate the enantiomers of pyrethroids such as cypermethrin etc.; the Supelcosil LC-CN column and the Chiralcel OD-H column were chained to separate the enantiomers of pyrethroids such as cypermethrin etc.. All diastereomers and enantiomers were sufficiently separated in a rather short time. The retention time and resolution of the diastereomers and enantiomers of pyrethroids were remarkably affected by the proportion of isopropyl alcohol in mobile phase. The resolution and retention time were increased while the proportion of isopropyl alcohol in mobile phase was decreased. There was no apparent change on the chiral recognization ability but observable change on the retention time when column temDerature was increased from 0 to 30℃.

关 键 词：高效液相色谱 拟除虫菊酯类农药 异构体 手性 

分 类 号：X502[环境科学与工程—环境工程]