蛇葡萄素对免疫细胞的趋化作用  

The Chemotaxis Effect of Ampelopsin on the Immunocytes

作　　者：曾飒[1] 罗高琴[1] 刘德育[1] 

机构地区：[1]中山大学药学院,广东广州510080

出　　处：《中药材》2006年第3期260-262,共3页Journal of Chinese Medicinal Materials

基　　金：广东省自然科学基金(20010762);广东省科技计划项目(2003C104033)

摘　　要：目的:观察蛇葡萄素在一定浓度下对中性粒细胞和单核细胞的趋化作用。方法:分别与趋化因子IL-8、MCP-1作对照,在琼脂糖中进行化学增活和化学趋化实验。结果:蛇葡萄素在25.6μg/m、l51.2μg/m l浓度下,具有显著诱导中性粒细胞和单核细胞产生趋化的作用。浓度为25.6μg/m l的蛇葡萄素与150 ng/m l IL-8或50 ng/m l的MCP-1的增活效应无显著差异(P>0.05)。浓度为12.8μg/m l的蛇葡萄素的趋化效应强于150 ng/m l的IL-8或100 ng/m l的MCP-1(P<0.05)。蛇葡萄素与IL-8及MCP-1对中性粒细胞和单核细胞的趋化作用均存在协同作用(P<0.05)。结论:蛇葡萄素对中性粒细胞和单核细胞均有化学增活和化学趋化效应,且在对免疫细胞的化学增活方面,蛇葡萄素与IL-8及MCP-1均存在协同作用。Objective： To study the chemotaxis effect of ampelopsin with different concentration on monocytes and neutrophilic granulocytes. Methods： Chemokinesis and chemotaxis tests were proceed in agarose gel comparing with chemokine IL-8 or MCP-1. Results： At 25.6 μg/ml or 51.2 μg/ml, ampelopsin could strongly enhance the migration of neutrophilic granulocytes and monocytes. The chemotaxis effect induced by 25.6 μg/ml of ampelopsin had no significant differences with that induced by 150 ng/ml of IL-8 or 50 ng/ml of MCP-1 （ P 〉 0. 05）. At a concentration of 12. 8 μg/ml, the chemokime effect of ampelopsin was more potent than that of 150 ng/ml of IL-8 or 100 ng/ml of MCP-1 （ P 〈0. 05）. Ampelopsin exerted a synergistic action with IL-8 or MCP-1 on its chemotaxis effect to neutrophilic granulocytes and monocytes. Conclusion： Ampelopsin can strongly enhance the chemokinesis and ehemotaxis effects of neutrophilic granulocytes and moncytes and exert a synergistic action with IL-8 or MCP-1 on its chemotaxis effect to neutrophilic granulocytes and monocytes.

关 键 词：蛇葡萄素 趋化作用 免疫细胞 IL-8 MCP-1 

分 类 号：R285.5[医药卫生—中药学]