超声波萃取-高效液相色谱法在海洋沉积物样品多环芳烃分析中的运用  

Determination of PAHs of Marine Sediments by HPLC Following Ultrasonic Extraction

作　　者：黄宏[1] 黄长江[1] 陈图锋[2] 朱爱意[3] 

机构地区：[1]汕头大学海洋生物研究所,广东汕头515063 [2]汕头大学分析测试中心,广东汕头515063 [3]浙江海洋学院海洋科学与技术学院,浙江舟山316042

出　　处：《海洋技术》2006年第1期67-72,共6页Ocean Technology

基　　金：国家自然科学基金资助项目(40176029);广东省重大科技兴海项目(A20000502F02)

摘　　要：建立用超声波萃取-高效液相色谱法同时检测海洋沉积物中16种优先监控的多环芳烃（PAHs）的方法。本方法采用丙酮作为萃取溶剂，在超声波作用下萃取出泥样中的PAHs，萃取液经浓缩后直接进行高效液相色谱分析。经对色谱条件和萃取条件进行优化后。各PAH组分的线性范围为1～500ng之间，相关系数0．9921～0．999，检出限0．1～4．6ng．达到海洋沉积物PAHs的检测要求。运用本法对粤东大规模增养殖区柘林湾表层沉积物PAHs进行检测。各PAH组分加标回收率为69．2％～108％，总平均达89．2％．相对标准偏差为0．5％～10．3％。本方法只需要萃取、浓缩和定容三个步骤，具有简便、迅速、高效、分析成本低的特点。A method using ultrasonie extraetion combined with separation by HPLC was established for the simultaneous determination of 16 PAHs in marine sediments. The PAHs （Polycyclic Aromatic Hydrocarbons） in the sediments were extracted by acetone in an ultrasonic bath and directly determinated by high performance liquid chromatography after concentrated in which the clean-up step is not necessary. After the chromatographic conditions and ultrasonic extraction were optimized, for each PAH component the linear limit ranges from 1 to 500 ng, the cofficient of correlation ranges from 0. 9921 to 0. 9999, and the detection limit ranges from 0.1 to 4.6 ng, which are up to scratch for marine sediment. Using the established method to measure the PAHs of sediments from Zhelin Bay of Eastern Guangdong with large-scale mariculture, the recoveries of PAHs ranged from 69.2% to 108% with a total average of 89.2% and standard deviations from 0. 5 to10. 3. The method is simple, fast, highly active, and low cost.

关 键 词：多环芳烃(PAHs) 海洋沉积物样品 超声波萃取 高效液相色谱(HPLC) 

分 类 号：P734.4[天文地球—海洋化学]