基于静电吸附甲苯胺蓝和纳米金固定过氧化物酶生物传感器的研究  

Hydrogen Peroxide Biosensor Based on Toluidine Blue and Nano-Au Through Electrostatic Adsorption

作　　者：黎雪莲[1] 袁若[1] 柴雅琴[1] 张凌燕[1] 王娜[1] 朱强[1] 

机构地区：[1]西南大学化学化工学院重庆市现代分析化学重点实验室,重庆400715

出　　处：《分析化学》2006年第3期389-392,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.29705001);重庆市自然科学基金(No.CSTC-2004BB4149);西南大学博士基金(No.SWNUB2004021)资助项目。

摘　　要：以玻碳电极为基底,电聚合邻氨基苯甲酸（ABA）,使其形成带负电的界面,通过分子间静电作用力,自组装一层带正电荷的电子媒介体甲苯胺蓝（TB）,再通过媒介体的氨基吸附纳米金,最后静电吸附固定辣根过氧化物酶制备出H2O2传感器.探讨了膜聚合时间、媒介体组装时间、pH、温度、工作电位对电极响应的影响.在优化的实验条件下,该传感器对H2O2电流响应与其浓度为1.5×10^-5～1.3×10^-3 mol/L范围内呈线性关系;检出限为5.6×10^-6mol/L.此外,该传感器具有较低的工作电位,能有效地消除抗坏血酸等的干扰.A novel approach by means of self-assembled technique and opposite-charged adsorption to immobilize horseradish peroxidase （HRP） to fabricate amperometric biosensor. The electrode architecture was fabricated by positively charged toluidine blue coated on negatively charged poly-2-aminobenzoic acid （PABA） modified glassy carbon electrode surface through electrostatic interactions to form a composite TB/PABA membrane, which yielded an interface containing amine groups to assemble gold nanoparticles for immobilization of HRP. The effects of experimental conditions such as the polymerization time of PABA, assembling-time for mediator, pH, temperature and applied potential on the electrode performance have been investigated. The linear response of the sensor to H2O2 iS in the range of 1.5×10^-5～1.3×10^-3 mol/L with a detection limit of 5.6×10^-6mol/L in0.1 mol/L PBS （pH 6.5） at -0.25 V. The current response time is 10 s. The biosensor retained 83.6% of its original activity after three weeks of use. Moreover, the studied biosensor exhibited good reproducibility, selectivity and high sensitivity.

关 键 词：邻氨基苯甲酸 甲苯胺蓝 纳米金 辣根过氧化物酶 自组装 化学修饰电极 

分 类 号：TP212.2[自动化与计算机技术—检测技术与自动化装置]