固相萃取-高效液相色谱法测定水体中苄嘧磺隆的残留量  

Determination of Bensulfuron-Methyl Residues in Water by High Performance Liquid Chromatography with Solid Phase Extraction

作　　者：岳霞丽[1] 张新萍[1] 董元彦[1] 

机构地区：[1]华中农业大学理学院,武汉市430070

出　　处：《光谱实验室》2006年第2期321-323,共3页Chinese Journal of Spectroscopy Laboratory

基　　金：国家自然科学基金资助课题(49831005)

摘　　要：建立了固相萃取-高效液相色谱(SPE-HPLC)测定水体中苄嘧磺隆残留量的方法。实验确定了固相萃取方法,优化了萃取条件,有效去除干扰成分。高效液相色谱采用EclipseXDB-C18(4.6×150mm,5μm)柱分离,以甲醇∶水∶冰醋酸(70∶29.5∶0.5,V∶V)为流动相,紫外检测波长为250nm。结果表明该法简便、快速、准确、灵敏度高,能满足水体中苄嘧磺隆残留分析要求。Bensulfuron-methyl residues in water was determined by high performance liquid chromatography （HPLC） with solid phase extraction （SPE）. The techniques of SPE including the activation of the SPE column, elution conditions of samples and elimination of interference were optimized. The separation was performed in a Eclipse XDB-C18 （4. 6mm i. d × 250mm, 5μm） and methanol,water and acetic acid as mobile phase,and detected at 250nm. The method is convenient, rapid,sensitive and accurate,and was used to analyze the residues of Bensulfuron-methyl in water.

关 键 词：固相萃取 高效液相色谱 苄嘧磺隆 残留量. 

分 类 号：O657.72[理学—分析化学]