饲养方式对鸡纹状体神经细胞形态的影响研究  

Morphological Changes of Chicken Dendritic Spine Associated with Different Rearing Conditions

作　　者：陈立波[1] 

机构地区：[1]青岛大学医学院,山东青岛266021

出　　处：《中国医学物理学杂志》2006年第2期118-120,122,共4页Chinese Journal of Medical Physics

摘　　要：目的:研究不同饲养方式对鸡新纹状体(MNH)神经细胞树突棘的影响。方法:选择刚孵出的WhiteLeghorn鸡18只,随机分为三组,每组6只,对照组(soc7day)同笼群养7天,(iso4day)组隔离单笼饲养4天,(iso7day)组隔离单笼饲养7天,分别于第4天和第7天将其处死并灌注固定,采用5℅LuciferYellow对MNH神经细胞进行细胞内染色,并用共聚焦激光扫描显微镜进行扫描观察,对神经细胞树突棘进行分类、测量和统计分析。结果:(iso4day)组中各种树突棘Dn、L、Dh的平均值均较对照组大(t=6.99;16.70;19.7均﹥2.58,p<0.01);树突棘密度(n/100μm)在中间段树突上和全部树突段上与(iso7day)组比较较小,差异有显著性(U=2.12,2.33均﹥1.96;p﹤0.05.)。(iso7day)组与(soc7day)组比较,各种树突棘Dn、T型树突棘长度L、S型和M型树突棘茎径Dn平均值均较对照组大,差异均有极显著性(t=12.28;9.03;10.63;9.02均﹥2.58,p﹤0.001.)。结论:社会接触和相互学习能影响脑MNH神经细胞初期的发育成长,提高其兴奋性突触后电位,提高突触间的传递效率,并能促使M型、S型树突棘向T型转化。Objective： To study the effects of different rearing conditions on the shape of dendritic spines. Methods： 18 newly hatched white Leghorn chicks were divided into 3 groups. The chicks were sacrificed at 4 and 7 days and fixed. 5% Lucifer Yellow was used for intracellar staining. MNH cells were observed under CLSM and analysed statistically. Results： The mean diameter of dendrite Dn,L ,Dh in iso 4day group was smaller than that of soc 7day group （t=6.99;16.70：19.7 〉 2.58,p〈0.01 ）; The mean values of spine frequency in the medial segment and entire dendrite in iso 4day group were smaller than that of iso 7day group（U=2.12,2.33 〉 1.96：p 〈 0.05.）, The mean diameter of dendrite neck and the diameter of dendrite of M-spine and S-spine as well as the length of T-spine in iso 7day group were significantly greater than that of soc 7day group （t=12.28 ;9.03 ; 10.63 ; 9.02 〉 2.58,p〈 0.001.）. Conclusion： Social contact can stimulate the growth of MNH neural cells, improve the excitatory postsynaptic potential and the transmission efficiency among synapses and stimulate the convertion of M-spine, S- spine to T-spine.

关 键 词：树突 树突棘 新纹状休 鸡 

分 类 号：R338.1[医药卫生—人体生理学]