雌激素诱导大鼠催乳素瘤发生机制的研究  

Mechanism of estrogen in uinduction of rat prolactinoma

作　　者：徐春[1] 刘亚力[1] 王莉[1] 侍晓云[1] 

机构地区：[1]武警总医院内分泌科,北京100039

出　　处：《武警医学》2006年第3期171-173,共3页Medical Journal of the Chinese People's Armed Police Force

基　　金：首都医学发展科研基金(中医药类)赞助项目(编号2001-Ⅲ-21);武警总医院科研基金(WZ200517)

摘　　要：目的研究雌激素诱发大鼠催乳素(prolactin PRL)瘤的机制。方法制备雌激素诱发的大鼠PRL瘤模型。放免法测定大鼠血清PRL水平。用免疫组化方法显示垂体组织PRL蛋白的表达和分布,用RT-PCR方法检测癌基因c-fos mRNA在各组垂体组织中的表达,以β-actin作为内参照,借助于计算机凝胶成像系统分析表达量。结果用药8周后,根据大鼠垂体重量,以及组织学和免疫组化的改变,证实已诱发出PRL瘤模型。在PRL瘤组中,c-fos的表达量明显高于对照组(P<0.01)。结论雌激素刺激垂体PRL细胞表达c-fos癌基因,在雌激素诱导PRL瘤中起一定的作用。Objective To study the mechanism of estrogen in inducing rat prolactinoma, Methods Adult Wistar rats were divided into two groups at random. Rat in control group（ n = 5）were subscutaneously implanted with a blank implant. Rats in prolactinoma group（ n = 5） were implanted with estrogen - containing implants. After 8 weeks, all the animals were sacrificed. Each pituitary gland was weighed and collected for histopathologlcal and immunocytochemical study. Serum prolactin（PRL） levels were measured by RIA. c - fos mRNA levels in pituitary tissue were measured by RT- PCR. Results Pituitary weights, PRL levels and PRL（ ＋ ） cell counts in prolactinoma group were higher than those in control group（ P 〈 0.1 ）, Prolactinoma models were developed successfully according to serum PRL level, pituitary weight and immunocytochemical profile. The expression levels of c - fos mRNA in prolactinoma group were obviously higher than those in control group（ P 〈 0.01 ）. Conclusion Estrogen stimulates c - fos gene expression, which plays an important role in induction of rat prolactinoma.

关 键 词：癌基因 c—fos 大鼠 催乳素瘤 雌激素 

分 类 号：R714.255[医药卫生—妇产科学] R295.5[医药卫生—临床医学]