固相萃取和高效液相色谱法测定鱼腥草中的黄酮  

Study on determination of flavonoid in houttuynia cordata thunb by solid phase extraction and RP-HPLC

作　　者：杨亚玲[1] 周强[1] 刘东辉[1] 赵榆林[1] 

机构地区：[1]昆明理工大学生命与化学工程学院,云南昆明650224

出　　处：《云南大学学报（自然科学版）》2006年第2期157-160,共4页Journal of Yunnan University(Natural Sciences Edition)

基　　金：教育部重点项目资助(地方02164)

摘　　要：研究了用固相萃取预分离,高效液相色谱法测定鱼腥草根和鱼腥草叶中黄酮类物质的方法.鱼腥草根和鱼腥草叶中的黄酮用80%乙醇溶液加热回流提取,提取液用Waters Sep-Pak-C18固相萃取小柱预分离脱脂,以Waters Nova-Pak-C18（φ3.9 mm×150 mm,5μm）色谱柱为固定相,以0.05 mol/L磷酸二氢钾缓冲溶液和甲醇的体积比为40：60的混合液为流动相,在该色谱条件下,鱼腥草中主要的黄酮成分均达到基线分离;用紫外二极管矩阵检测器在360nm波长处检测,并作了色谱峰纯度分辨.方法标准回收率为98%～101%,相对标准偏差为0.85%～2.2%.A high performance liquid chromatography （PIPLC） method for the determination of flavonoid in myrica rubra leaf and bark was studied. The flavonoid was extracted from the sample with 80% of ethanol by heating circumfluence. Then the extract was degreased by solid phase extraction with Sep - Pak - C18 cartridge. The flavonoid was separated on a Nova - Pak - C-48 chromatogram column （3.9 × 150 mm, 5μm）, with methanol and 0.05 mol/L potassium dihydrogen phosphate buffer solution as mobile phase at 60： 40 （V/V）, and monitored with the photodiode array detector at 360 nm. The recoveries of the flavonoid are 98%- 101%, and relative standard deviations are 0.85 %-2.2 %.

关 键 词：高效液相色谱 固相萃取 鱼腥草 黄酮 

分 类 号：O657.7[理学—分析化学]