红花长寿花愈伤组织的诱导和植株再生研究  

Callus induction and Plant Regeneration in Kalanchoe Blossfeldiana with Red Flowers

作　　者：黄双龙[1] 赖钟雄[1] 林秀莲[1] 林玉玲[1] 万学锋[1] 吴金寿[1] 柯存祥[2] 

机构地区：[1]福建农林大学园艺植物生物工程研究所,福州350002 [2]福建农林大学园艺学院,福州350002

出　　处：《中国农学通报》2006年第3期48-52,共5页Chinese Agricultural Science Bulletin

基　　金：农业部948计划资助项目"植物保鲜相关基因的引进及其在亚热带园艺作物上的应用"(No.991029)。

摘　　要：试验采用红花长寿花茎段和叶片作为外植体,在附加不同植物激素组合的培养基中对愈伤组织的诱导和植株再生进行研究。结果表明:不带叶柄的叶片在MS+2,4-D1.0mg/L+BA0.5mg/L的培养基上对愈伤组织的诱导和增殖效果最好;在MS+BA0.25mg/L+2,4-D1.5mg/L+GA0.6mg/L的培养基上有利于愈伤组织转绿分化;在MS+NAA0.25mg/L+BA1.5mg/L+GA0.6mg/L的培养基上有利于丛生芽诱导和增殖;在1/2MS+NAA0.3mg/L+IBA0.7mg/L培养基中生根效果最好。The stem sections and the leaves from the plants were used as explants and the callus induction and plant regeneration were conducted on the media supplemented with different plant hormone combinations in Kalanchoe blossfeldiana with red flowers in this experiment. The obtained results showed that the MS medium supplemented with 1.0 mg/L 2,4- D and 0.5 mg/L BA was optimal for the callus induction and propagation from leaves without leafstalks; the MS medium supplemented with 0.25 mg/L BA and 1.5 mg/L 2,4- D and 0.6 mg/L GA was beneficial to the differentiation of green callus; and the MS medium supplemented with 1.5 mg/L BA and 0.25 mg/L NAA and 0.6mg/L GA was propitious to the induction and the propagation of bud clusters; and the highest percentage of rooting was obtained on the 1/2 MS medium adding 0.3 mg/L NAA and 0.7 mg/L IBA.

关 键 词：红花长寿花 愈伤组织 植株再生 

分 类 号：S681[农业科学—观赏园艺]