转枯草芽孢杆菌纤溶酶基因对烟草激素代谢的影响  

Metabolism Variation of Plant Hormones in BSFE Transgenic Tobacco

作　　者：李国婧[1] 王金祥[1] 王瑞刚[1,2] 吴自荣[3] 王水平[3] 

机构地区：[1]内蒙古农业大学生物工程学院 [2]华东师范大学生命科学学院,上海200062 [3]华东师范大学生命科学学院

出　　处：《中国农学通报》2006年第3期76-79,共4页Chinese Agricultural Science Bulletin

基　　金：上海市科委重大项目"安舒克栓酶的药效及工艺研究"(985419000-3)。

摘　　要：利用酶联免疫(ELISA)检测和分析了生长6、12、18、24周龄的转枯草芽孢杆菌纤溶酶(Bacil-lussubtilisfibrinolyticenzyme,BSFE)基因烟草与野生型烟草的内源ZRs、IAA、GA1+3、ABA水平。结果表明,转基因烟草与野生型烟草的激素代谢规律基本一致,但在激素水平上存在明显差异。表现为转基因烟草的IAA、GA1+3、ZRs水平较野生型烟草低,而ABA水平却高于野生型烟草。推测其于转基因烟草生长劣势有关。The endogenous ZRs, IAA, GA1＋3 and ABA level of BSFE transgenic tobacco and wild type were assayed by ELISA respectively. The results showed that in 6, 12, 18, 24 weeks old tobacco plants, the metabolism patterns of different plant hormones are the same in both transgenic and wild type, while the level of IAA, GAI^3 and ZRs in transgenic tobacco were lower than, and the level of ABA was higher than that in wild type. We infer that these differences in plant hormones level related with the growth inhibition of transgenic tobacco.

关 键 词：枯草芽孢杆菌纤溶酶 转基因烟草 ZRs IAA GA1+3 ABA ELISA 

分 类 号：S572[农业科学—烟草工业] Q936[农业科学—作物学]