GM-CSF/IL1-5融合基因原核表达载体的构建及表达  

Clone and Expression of A Recombinant Vector for GM-CSF/IL-15 Fusion Gene

作　　者：谢云青[1] 郑天荣[1] 郑秋红[1] 汪相如[1] 龚福生[1] 陈蓉明[1] 

机构地区：[1]福建医科大学教学医院,福建省肿瘤医院分子生物学研究室,福州350014

出　　处：《福建医科大学学报》2006年第2期111-113,共3页Journal of Fujian Medical University

基　　金：福建省卫生厅医学创新基金资助项目(2001CX12)

摘　　要：目的构建原核表达质粒pPROEXTMHTbGMCSF/IL15并在大肠杆菌DH5α中表达,以期获得具有GMCSF/IL15双重活性的融合蛋白。方法利用分子生物工程技术,构建重组原核表达载体pPROEXTMHTbGM15,并转化进大肠杆菌DH5αIPTG诱导表达4h后,经SDSPAGE、免疫印迹证实为GM15融合蛋白。结果重组载体经转化大肠杆菌DH5α后,诱导表达出相对分子质量约为33kD左右的融合蛋白。结论构建了pPROEXTMHTbGMCSF/IL15融合基因表达系统,并诱导表达出GM15融合蛋白。Objective Construct pPROEXTM HTb-GM-CSF/IL-15 and express in DH5α to obtain the GM-15 fusion protein, which may have double activity. Methods Using molecular biological techniques, a recombinant vector pPROEXTMHTb-GM-CSF/IL-15 was constructed, and express fusion protein inducing by IPTG 4 h. The fusion protein was vetified by SDS-PAGE, Western blot. Results The fusion protein with relative molecular mass of 33 kD was yielded in DH5α by induced. If possessed double activity of anti GM-CSF and anti IL-15 by western blot assay. Conclusion The successful construction of the expression vector laid a foundation of largly product and clinical use of the fusion protein.

关 键 词：GM—CSF 白细胞介素15 重组融合蛋白质类 质粒 基因表达 

分 类 号：Q78[生物学—分子生物学]