Intra-specific genetic relationship analyses of Elaeagnus angustifolia based on RP-HPLC biochemical markers  

作　　者：WANG Qiang RUAN Xiao HUANG Jun-hua XU Ning-yi YAN Qi-chuan 

机构地区：[1]Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, China [2]School of Forestry, Xinjiang Agriculture University, Urumqi 830052, China [3]School of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2006年第4期272-278,共7页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：Project (Nos. 30470330 and 30100126) supported by the NationalNatural Science Foundation of China

摘　　要：Elaeagnus angustifolia Linn. has various ecological, medicinal and economical uses. An approach was established using RP-HPLC （reversed-phase high-performance liquid chromatography） to classify and analyse the intra-specific genetic relationships of seventeen populations of E. angustifolia, collected from the Xinjiang areas of China. Chromatograms of alcohol-soluble proteins produced by seventeen populations of E. angustifolia, were compared. Each chromatogram of alcohol-soluble proteins came from a single seed of one wild plant only. The results showed that when using a Waters Delta Pak. C 18, 5μm particle size reversed phase column （150 mm×3.9mm）, a linear gradient of 25%-60% solvent B with flow rate of 1 ml/min and run time of 67 min, the chromatography yielded optimum separation of E. angustifolia alcohol-soluble proteins. Representative peaks in each population were chosen according to peak area and occurrence in every seed. The converted data on the elution peaks of each population were different and could be used to represent those populations. GSC （genetic similarity coefficients） of 41% to 62% showed a medium degree of genetic diversity among the populations in these eco-areas. Cluster analysis showed that the seventeen populations orE. angustifolia could be divided into six clusters at the GSC=0.535 level and indicated the general and unique biochemical markers of these clusters. We suggest that E. angustifolia distribution in these eco-areas could be classified into six variable species. RP-HPLC was shown to be a rapid, repeatable and reliable method for E. angustifolia classification and identification and for analysis of genetic diversity.

关 键 词：Elangustifolia Intra-specific genetic relationship Genetic diversity Biochemical marker 

分 类 号：Q94[生物学—植物学]