乙型肝炎疫苗-卡介苗联合疫苗细胞免疫的研究  

Cellular Immunity of Hepatitis B-BCG Combined Vaccine

作　　者：金立杰[1,2] 赵小琳[1] 方艳秋[3] 杨丽华[1] 王志[1] 

机构地区：[1]长春生物制品研究所 [2]吉林大学生命科学院,长春130021 [3]吉林大学第一医院

出　　处：《中国生物制品学杂志》2006年第2期171-173,共3页Chinese Journal of Biologicals

基　　金：吉林省科技发展计划项目(20050402-1).

摘　　要：目的评价乙型肝炎疫苗-卡介苗联合疫苗对小鼠细胞免疫的影响。方法采用BALB/c小鼠,试验组分为联合疫苗组、卡介苗组、乙肝疫苗组,对照组分为空白组和保护剂组,各组小鼠分别于接种后1个月及2个月,取脾进行淋巴细胞转化试验以及细胞因子和T淋巴细胞表面标记检测。结果联合疫苗组淋巴细胞转化刺激指数、IL-2含量均明显高于卡介苗组和乙肝疫苗组;CD4+/CD8+值高于单价乙肝疫苗组,而与卡介苗组差异无显著意义。结论乙型肝炎疫苗-卡介苗联合疫苗能有效地诱导细胞免疫。Objective To evaluate the influence of Hepatitis B （HB）-BCG combined vaccine on the cellular immunity in mice. Methods Divide BALB/c mice into three test groups （ HB-BCG combined vaccine, BCG and HB vaccine）, one blank control group（unimmunized） and one parallel control group （protective agent）. Take the spleens of mice for lymphocyte transformation test and the determination of cytokine and T lymphocyte surface marker 1 and 2 months after immunization separately. Results Both the lymphocyte transformation stimulating index and IL-2 content of mice in HB-BCG combined vaccine group were significantly higher than those of BCG and HB vaccine groups. However, the CD4^＋/CD8^＋ value of HB-BCG combined vaccine group was significantly higber than that of HB vaccine group,but showed no significant difference from that of BCG group. Conclusion HB-BCG combined vaccine induced cellular immunity effectively.

关 键 词：乙型肝炎疫苗 卡介苗 联合疫苗 细胞免疫 

分 类 号：R512.62[医药卫生—内科学] R392.1[医药卫生—临床医学]