β-环糊精修饰区带毛细管电泳法测定芳香族羧酸  

Determination of acromatic carboxylic acids by β-cyclodextrin modified capillary zone electrophoresis

作　　者：吕元琦[1] 李新民[1] 周连文[1] 华玉山[1] 张智勇[2] 

机构地区：[1]德州学院分析测试中心,山东德州253023 [2]中国科学院高能物理研究所核分析技术重点实验室,北京100049

出　　处：《化学工程师》2006年第3期21-22,25,共3页Chemical Engineer

基　　金：中国科学院核分析技术重点实验室基金资助项目(No.K-118)

摘　　要：建立了一种β-环糊精修饰区带毛细管电泳法分析8种芳香族羧酸的方法。在优化的条件下，8种物质在12min内得到良好分离。苯甲酸、2，4-二羟基苯甲酸、2，5-二羟基苯甲酸、3，4-二羟基苯甲酸、肉桂酸、2-硝基苯甲酸、3-硝基苯甲酸和4-硝基苯甲酸峰面积和质量浓度分别在2—100、1～100、2-100、1～100、2．2-110、4-200、2-100和2．4-120μg·mL^-1范围内成良好线性。检出限分别为0．2、0．2、0．3、0．2、0．5、0．8、0．3和0．4μg·mL^-1。该方法被用于模拟样品分析，所得结果和真实值一致。A method has been established to determine 8 kinds of acromatic carboxylic acids by β- cyclodextrin modified capillary zone electrophoresis. Under the optimized conditions, the 8 kinds of analytes were well separatod in 12min. A good linear relationship existed between peak area and concentration in the 2 - 100,1 - 100,2 - 100,1 - 100, 2.2- 110,4 - 200,2 - 100 and 2.4 -120μg·mL^-1 concentration range for benzoic acid, 2,4 - dihydroxybenzoic acid, 2,5 - dihydroxybenzoic acid, 3,4 - dihydroxybenzoic acid, cinnamic acid, 2 - nitrobenzoic acid, 3 - nitrobenzoic acid and 4- nitrobenzoic acid, respectively. The detection limits, based on three times noise were 0.2,0.2,0.3,0.2,0.5, 0.8,0.3 and 0.4μg·mL^-1, respectively. The developed method was applied to simulated sample analysis, the results were in good agreement with the real value.

关 键 词：区带毛细管电泳(CZE) β-环糊精(β-CD) 芳香族羧酸 

分 类 号：TQ151.7[化学工程—电化学工业]