消痞颗粒对实验大鼠慢性萎缩性胃炎伴不典型增生胃黏膜PCNA表达的影响  

Influence of the Xiaopi Granules on the Expression of PCNA in Gastric Mucosa of the Rats with CAG Accompanied by Dyspalsia

作　　者：孟捷[1] 杨晋翔[1] 鲁香凤[2] 戴欣[2] 方杰[2] 

机构地区：[1]北京中医药大学东方医院,北京100078 [2]北京中医药大学东直门医院病理科,北京100700

出　　处：《辽宁中医杂志》2006年第3期369-371,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：教育部科学技术研究重点项目资助(01031)

摘　　要：目的:从胃黏膜PCNA表达变化的角度进一步探讨实验性慢性萎缩性胃炎伴不典型增生发病机理及消痞颗粒对其治疗作用的机制。方法:将大鼠随机分为5组,采用综合法(幽门弹簧插入加高盐热糊法)复制大鼠慢性萎缩性胃炎伴不典型增生模型,免疫组化法显示胃黏膜PCNA表达。结果:模型组及自然恢复组大鼠胃黏膜PCNA阳性表达明显增强,消痞颗粒治疗组PCNA表达则明显减弱(P<0.05)。结论:消痞颗粒对CAG大鼠胃黏膜活跃的增殖状态有明显抑制作用,可能是消痞颗粒治疗慢性萎缩性胃炎伴不典型增生有效机制之一。Objective： To explore the pathogenesis of experimental chronic atrophic gastritis with dysplasia and treatment mechanisms of Xiaopi granules from the view point of the change of the expression of PCNA of gastric mucosa. Methods： The Wistar rats were randomly divided into 5 groups. The CAG model was made by using a synthetic method, a combined application of insertion of a metallic spring into the gastric pylorus, oral administration of hot salty paste（60 - 70℃, 10% NaCl）twice a week, on the basis of the model, the expression of PCNA of the gastric mucous in the rats were displayed by immunohistological techniques. Results ： The positive expression of PCNA in gastric mucosa of the rats in model group and spontaneous recovery group was significantly higher than in normal group, The positive expression of PCNA was significantly decreased after treated with Xiaopi granule, there was siginificant difference between spontaneous recovery group and Xiaopi granule group. No significant difference showed between control group treated with Weimeisu. Conclusion：Inhibiting the positive expression of PCNA may be one of the mechanisms of Xiaopi granule＇ s treatment effects on CAG accompanied by dysplasia.

关 键 词：消痞颗粒 慢性萎缩性胃炎 不典型增生 PCNA 增殖 

分 类 号：R285.5[医药卫生—中药学]