SARS-CoV感染布氏田鼠、Lewis大鼠和恒河猴引起的病理变化  

Pathological changes on SARS-CoV infected Microtus brandtii, Lewis rat and Rhesus macaque

作　　者：秦川[1] 邓巍[2] 张连峰[2] 丛斌[1] 

机构地区：[1]河北医科大学,河北石家庄050071 [2]中国医学科学院中国协和医科大学实验动物研究所,北京100021

出　　处：《基础医学与临床》2006年第3期261-265,共5页Basic and Clinical Medicine

基　　金：国家高技术研究发展计划项目(2003AA038213)

摘　　要：目的研究SARS-CoV感染布氏田鼠、Lewis大鼠和恒河猴后引起的病理学、免疫学变化及病毒复制与外排情况变化特点及作为动物模型的可行性。方法SARS病毒感染布氏田鼠、Lewis大鼠和恒河猴,用病毒分离和RT-PCR方法检测病毒复制与外排;用ELISA检测特异性抗体;用光学显微镜对动物的各脏器进行病理观察研究。结果在SARS-CoV感染的3种动物肺组织均出现与人类SARS疾病相似的病理改变,均可检测到病毒核酸和特异性IgG抗体的存在。结论恒河猴、Lewis大鼠和布氏田鼠感染SARS-CoV后,均能在一定程度上再现人类SARS患者的病理特征,其中恒河猴的病理改变更近似于人类患者,故以恒河猴为模型可能对研究SARS发病机制和疫苗评价更为理想。Objective The pathological changes on SARS CoY infected Microtus brandtii, Lewis rat and Rhesus macaque and the possibility as SARS animal model were studied. Methods The amimals were infected with SARS-CoV through nasal cavity and sacrificed for collecting pathologic tissues after inoculation. The pathological changes, immuno-response and the replication of SARS-CoV in vivo were tested with microscopy, ELISA and virus isolation. Results Similar pathological changes were observed in SAILS CoV infected Rhesus macaques, Lewis rats and Microtus brandtii and the pathological changes were similar with those of human. Conclusion All of the tree species of animals infected with SARS-CoV showned similar pathological changes with human SARS case. Among them, SARS-CoV infected Rhesus macaques is the best one in terms of pathological changes. Therefor the infected Rhesus macaque may be one of the most ideal animal model to study the pathogenesis and evaluation of anti-SARS-CoV vaccine.

关 键 词：严重急性呼吸综合症 恒河猴 LEWIS大鼠 布氏田鼠 动物模型. 

分 类 号：R511.9[医药卫生—内科学]